NDV7793体外激活的小鼠单核巨噬细胞(MΦ)对小鼠肝癌细胞的杀伤作用及其机制

Killing Effect on Liver Cancers by Mouse Macrophages Stimulated by Newcastle Disease Virus 7793 Strain in vitro and Its Mechanism

目的初步研究NDV7793激活的小鼠单核巨噬细胞(MΦ)对小鼠肝癌Novikoff细胞的杀伤作用,并探讨其杀伤机制与TNF-α和TRAIL的关系。方法从腹腔分离6周龄BALB/C小鼠MΦ,用NDV7793于体外刺激小鼠MΦ,以ELISA分别测定NDV7793刺激小鼠MΦ后产生的TNF-α及TRAIL水平;NDV7793体外刺激MΦ后,与小鼠肝癌Novikoff细胞混合培养,以LDH微量释放法测定小鼠MΦ对小鼠肝癌Novikoff细胞的杀伤效应。同时设立3组实验对照组:IFN-β阳性对照组、紫外线灭活NDV(UV-NDV)对照组以及空白对照组。结果与3个对照组相比,NDV7793在体外能提高MΦ分泌TNF-α、TRAIL的水平;NDV体外刺激后的小鼠MΦ能杀伤小鼠肝癌Novikoff细胞。结论NDV7793在体外能激活小鼠MΦ,小鼠MΦ被NDV7793刺激后,对小鼠肝癌Novikoff细胞的杀伤作用增强,NDV激活后的小鼠MΦ对小鼠肝癌Novikoff细胞的杀伤机制可能与TNF-α和TRAIL有关。

Objective To study the killing effect on liver cancers by mouse macrophages stimulated by Newcastle disease virus 7793 strain in vitro and the association of TRAIL.Methods The BALB/C mouse macrophages were harvested by using peritoneal lavage.And then the mouse macrophages were stimulate in vitro by NDV7793.The concentration of TNF-α and TRAIL was determined by ELISA after NDV stimulation.Then the macrophages of mice were coincubated and activated with Novikoff cells.The cytotoxic effect of macrophages on Novikoff cells was performed by Lactate Dehydrogenase（LDH） assay after NDV stimulation.Three experiment control groups were simultaneously set up as following：IFN-β positive control group,ultraviolet ray inactivated NDV（UV-NDV） control group as well as blank control group.Results Compared with three control groups in vitro,the macrophages stimulated with NDV 7793 had been activated,and the level of TNF-α and TRAIL in culture supernatant increased.The killing ability of macrophage to Novikoff cells after NDV stimulation had increased.ConclusionThe NDV 7793 can activate the mouse macrophages in vitro.The killing effect on liver cancer cells of the mouse macrophages is enhanced by NDV stimulation.And it is possible that TRAIL and TNF-α involve in this may enhance the killing effect.