UHPLC-Q-TOF/MS对藜芦药材化学成分的快速分离与鉴别

UHPLC-Q-TOF/MS in rapid separation and identification of chemical components in Veratrum nigrum L.

目的采用超高效液相色谱仪与四极杆飞行时间串联质谱仪联用技术(UHPLC-Q-TOF/MS)对藜芦中化学成分进行分析鉴别。方法应用Agilent Eclipse Plus C18色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相梯度洗脱,电喷雾离子源,正离子模式下采集数据。结果共鉴别出黎芦药材中32个化学成分,通过比较碎片离子对其中的3对同分异构体进行了结构解析。结论采用UHPLC-Q-TOF/MS方法能快速地在一张图谱上表征出藜芦中的主要化学成分,为藜芦的药效物质基础及化学成分体内代谢与作用机制的深入研究奠定了基础。

Objective To identify the chemical components in traditional Chinese herbal medicine Veratrum nigrum L.using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry（UHPLC-Q-TOF/MS）.Methods An Agilent Eclipse Plus C18（2.1 mm×100 mm,1.8 μm）was used for isolation and identification of the chemical components in Veratrum nigrum L.,with a mobile phase using 0.1% formic acid aqueous solvent（A） and ACN（B） for gradient elution.The data were collected by the positive ion mode using ESI ion source.Results Thirty-two chemical compounds were identified from Veratrum nigrum L..The chemical structures of three pairs of steroidal alkaloid isomers were deduced by comparing their fragment ions.Conclusion Chromatographic characters of 32 chemical compounds in Veratrum nigrum L.can be achieved in single run by UHPLC-Q-TOF/MS,which paves a way for further studying the metabolism and action mechanism of Veratrum nigrum L.