大鼠非梗死区心肌Dock1蛋白表达改变

Change of Dock1 protein expression in non-infarcted myocardium in rats

目的探讨大鼠非梗死区心肌Dock1蛋白的表达改变。方法建立SD大鼠实验性心肌梗死(22只)及假手术模型(20只)。分别于术后24 h和12周时处死心肌梗死组和假手术组大鼠(各8只),获取心脏标本,应用免疫印迹法检测非梗死区心肌及假手术组心肌Dock1蛋白的表达。结果术后24 h时,心肌Dock1蛋白表达在梗死组(0.13±0.03)与假手术组(0.10±0.04)间的差异无统计学意义(P>0.05);但术后12周时,梗死组(0.17±0.04)较假手术组(0.11±0.05)表达增加,差异有统计学意义(P<0.05);术后12周时的梗死组心肌Dock1蛋白表达较24 h时的梗死组和假手术组均增加,差异有统计学意义(P<0.05)。结论心肌中存在Dock1蛋白的表达;梗死后12周时非梗死区心肌Dock1蛋白表达增加。

Objective To investigate the change of Dock1 protein expression in non-infarcted myocardium in rats.Methods Experimental myocardial infarction models were established in 22 SD rats（MI group） and the other 20 rats received sham-operation（Sham group）.Eight rats were sacrificed 24 h after operation in each group and another 8 were sacrificed at 12 weeks after operation;the cardiac specimens were collected.Dock1 protein expression was measured by Western blotting analysis in the non-infarcted myocardium of MI group and sham group.Results Dock1 protein expression was not significantly different in the non-infarcted myocardium between MI group（0.13±0.03） and Sham group（0.10±0.04） at 24 h after operation（P0.05）;12 weeks after operation,the expression in non-infarcted myocardium of MI group（0.17±0.04） was significantly higher than that of Sham group（0.11±0.05,P0.05）.Dock1 protein expression in non-infarcted myocardium was significantly increased in MI group at 12 weeks after operation compared with those in MI and Sham groups at 24 h after operation（P0.05）.Conclusion There is Dock1 protein expression in rat＇s myocardium,and the expression is significantly increased in the non-infarcted myocardium at 12 weeks after infarction.