Ⅰ型雏鸭肝炎病毒侵染后鸭组织中ALB基因表达的分析

Analysis of Gene Expression of ALB in Duck Tissues Infected Duck Hepatitis Virus

检测Ⅰ型雏鸭肝炎病毒侵染后ALB基因mRNA以及ALB蛋白分别在肝脏、脾脏、肺脏、肾脏、大脑、小脑、腿肌和胸腺等组织中的相对表达量和含量并分析其意义。分别利用实时荧光定量RT-PCR技术和ELISA法检测ALB基因在易感组、抗病组和对照组各组织中mRNA的表达量以及ALB蛋白含量。总体而言,除腿肌外,ALB基因mRNA在易感组中的表达量极显著低于对照组和抗病组(P<0.01),抗病组显著或极显著低于对照组(P<0.01或P<0.05);各处理组间,肝、小脑和胸腺中ALB蛋白含量与ALB基因mRNA相对表达量规律一致,在其他组织中,各处理组间ALB蛋白含量差异均不显著(P>0.05)。RT-PCR与ELISA的结果比较可见,两者在肝脏、胸腺、小脑等与雏鸭肝炎病直接相关的指示性组织中表现一致。本试验研究结果,进一步揭示了ALB基因为Ⅰ型雏鸭肝炎病的抗性基因,与前期抑制性消减杂交法得到的结果一致,其表达量的变化可以作为区分易感鸭和抗病鸭的标志。

This research tried to detect the relative expression level of mRNA and protein of ALB gene in livers,pancreases,lungs,kidneys,cerebra,cerebella,leg muscles and thymuses infected by duckling hepatitis virus（DHV-1）.The expression level of ALB gene and the content of ALB protein of control group,susceptible group and resistant group were detected by real-time quantitative RT-PCR and ELISA techniques.Generally speaking,except in leg muscles,the expression level of ALB gene＇s mRNA in susceptible group was highly significantly less than those in control group and resistant group（P0.01）,and the level in resistant group was significantly or highly significantly less than that in control group（P0.05 or P0.01）.And the content of ALB protein has the same differences as ALB gene＇s mRNA in livers,cerebella and thymuses among those three groups,but there were no significant differences in the other tissues（P0.05）.It was showed that the results of RT-PCR and ELISA techniques had the same results in the indicative tissues directly connecting with duck hepatitis such as livers,thymuses,cerebella,etc.This research gets the same results as those using suppression subtractive hybridization（SSH） and makes a further step to reveal that ALB gene is the resistant gene of duck hepatitis,whose change of the expression level can be used as a marker to distinguish susceptible and disease-resistant ducks.