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N-乙酰半胱氨酸对氢醌抑制HL-60细胞分化的保护作用

Effect of N-acetylcysteine on the suppression of HL-60 cells differentiation induced by hydroquinone
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摘要 目的探讨N-乙酰半胱氨酸(NAC)在氢醌(HQ)抑制HL-60细胞向单核系分化过程中所起的作用。方法将NAC 1、2.5、5和10 mmol/分别与HQ 50μmol/L和豆蔻酰佛波乙酯(TPA)20 nmol/L共同处理细胞,处理48 h后收集细胞。通过硝基四氮唑蓝还原反应鉴定细胞分化;CCK-8检测细胞增殖;活性氧检测试剂盒鉴定活性氧的变化。结果与HQ和TPA+HQ组比较,随NAC剂量的增加可以引起细胞增殖的增加,差异具有统计学意义(P<0.01)。在HL-60细胞向单核系分化的过程中,与TPA+HQ组比较,细胞的分化能力随着NAC剂量的增加而增强,差异具有统计学意义(P<0.01),其中NAC 5和10 mmol/L作用最为显著。细胞内活性氧(ROS)检测显示,与空白对照组比较,HQ组和TPA+HQ组含量明显增强,差异有统计学意义(P<0.01);与TPA+HQ组比较,NAC各个剂量组ROS含量明显降低(P<0.01)。5个时间点测量细胞内活性氧含量差异有统计学意义(F=564.3,P<0.01)。结论 HQ能够抑制HL-60向单核系分化,这种抑制作用会随着NAC剂量的增加而降低,这种影响是通过清除细胞内活性氧来实现的。 Objective To study the role of N-acetylcysteine(NAC) in the process of inhibition of monocytic differentiation in HL-60 cells induced by hydroquinone(HQ) differentiated to monocytes.Methods HL-60 cells were co-treated with 1-10 mmol /L of NAC,50 μmol /L of HQ and 20 nmol /L of cardamom acid phorbol ester(TPA),and then cells were collected after cultured for 48 h.Differentiation was assessed by the reduction of nitro blue tetrazolium assay;cell proliferation was detected by CCK-8;the change of reactive oxygen species was assessed by reactive oxygen species test kit.Results Compared with the HQ group and TPA + HQ group,cell viability was enhanced with the addition of NAC,the difference was statistically significant(P0.01).In the process that HL-60 cells differentiated to monocytes,compared with the TPA+HQ group,cell differentiation was enhanced with the addition of NAC,the difference was statistically significant(P0.01),among which NAC 5 mmol/L and NAC 10 mmol/L had the most significant difference.ROS detection showed that,compared with the control group,the intracellular reactive oxygen species of the HQ group and the TPA+HQ group were significantly enhanced(P0.01);compared with the TPA+HQ group,the intracellular reactive oxygen species of NAC groups at all doses were decreased significantly(P0.01).At 5 different time points,the intracellular reactive oxygen species levels were significantly different,F=564.3,P0.01.Conclusion HQ can inhibit HL-60 differentiation to monocytes.This inhibition would decrease with the increasing of the NAC's dose,and this effect comes to achieve by clearing the intracellular reactive oxygen species.
出处 《毒理学杂志》 CAS CSCD 北大核心 2012年第2期94-97,共4页 Journal of Toxicology
基金 国家自然基金资助项目(30600505) 教育部科学技术研究重点项目(208107) 2009年度广西高校优秀人才资助计划
关键词 N-乙酰半胱氨酸 HL-60细胞 细胞分化 活性氧 N-acetylcysteine HL-60 cells Cell differentiation Reactive oxygen species
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