蛛网膜下腔出血后Caspase-12表达及海马细胞凋亡的研究

Research of the gene expression levels of Caspase - 12 and neuronal apoptosis in the hippocampus after subarachnoid hemorrhage

目的检测内质网特异性因子Caspase-12及海马区神经元细胞凋亡的表达，分析内质网应激在自发性蛛网膜下腔出血（SAH）后早期脑损伤中的作用。方法54只大鼠分为手术组、假手术组、空白对照组；手术组、假手术组又分为3h、12h、24h、48h亚组。利用lit—PCR检测Caspase-12的表达，TUNEL测大鼠海马区神经元细胞凋亡，电镜及HE染色光镜下观察大鼠海马区神经元细胞形态学改变。结果Caspase-12在SAH后3h开始增高，24h达到高峰，48h开始降低，但48h时较假手术组、空白对照组仍高（P〈0．01），各组比较差异有统计学意义（P〈0．01）。大鼠海马区神经元凋亡阳性细胞在3h时开始出现，但与假手术组、空白对照组相比差异无统计学意义（P〉0．05），凋亡阳性细胞数在24h达高峰，48h开始降低，但较假手术组、空白对照组仍高（P〈0．01）。各组间比较差异有统计学意义。手术组大鼠海马区神经元细胞在光镜下可见明显核固缩。各组间Caspase-12的表达量与细胞凋亡阳性数量呈正相关（r=0．753，P〈0．01）。结论内质网应激在SAH后早期脑损伤中发挥重要作用。

Objective To detect the gene expression levels of Caspase - 12 and neuronal apoptosis in the hippocampus of rats in order to analyze endoplasmic reticulum stress in early brain injury after subarachnoid hemorrhage . Methods The gene expression levels of Caspase - 12 in the 54 rats were detected by Real -Time PCR. The neuronal apoptosis in the hippocampus was detected by TUNEL. To observe change of neuron of hippocampus area by light microscope and electron microscope. 54 rats were divided into operation group, sham group and control group. The operation group was divided into 3 hours time group, 12 hours time group, 24 hours time group and 48 hours time group. Results The gene expression levels of Caspase - 12 were significantly higher in the 3 hour time quantum than in the sham group （ P 〈 0. 01 ） and control group （ P 〈 0. 01 ） , reach the peak in 24 hour time quantum , began to down in the 48 hour time quantum but were higher than sham group and control group. The neuronal apoptosis were find in the 3 hour time quantum but the number of neuronal apoptosis was no difference than sham group and sham group（ P 〉 0. 05）. The number of neuronal apoptosis reached the peak in 24 hour time quantum, began to clown in the 48 hour time quantum. But the number of neuronal apoptosis in the 48 time quantum was higher than sham group and control group （ P 〈 0. 01 ）. By correlate analysis, the gene expression levels of Caspase - 12 and the number of neuronal apoptosis were indicated the most close correlation（ r =0. 753, P 〈 0.01 ）. Conclusion Endoplasmic reticulum stress plays an important role in early brain injury after subarachnoid semorrhage.