摘要
目的通过对人脑胶质瘤U87细胞中CD133＋和CD133一细胞进行配对研究，寻找差异性蛋白。方法通过磁珠选择分离U87中CD133＋和CD133-细胞，利用双向凝胶电泳（2D—PAGE）检测上述细胞总体蛋白，分析差异性蛋白点。通过质谱（MALDI—TOF—MS）对蛋白点进行鉴定，建立两种细胞差异性蛋白文库。结果CD133＋和CD133一细胞配对比较，蛋白质组学检测结果中，差异有统计学意义（P〈0．05）的2D—PAGE蛋白点共有73个，〉1．5倍差异蛋白点有46个，〉2倍差异蛋白点有27个，〉3倍差异蛋白点有8个。在CD133＋U87细胞中，有10个蛋白表达增多（上调），63个蛋白表达下降（下调）。共得到44张高质量质谱肽质量指纹图谱（PMF），鉴定了35种蛋白质。结论CD133＋和CD133-细胞是同一细胞株中两种不同的细胞，蛋白方面存在的差异，将成为进一步研究它们相互关系的重要靶点。
Objective To analyze differences between CD133＋ and CD133- glioma U87 cells by methods of proteomics. Methods 2D - PAGE was performed to separate the proteins of the CD133 ＋ and CD133- U87 cells, which were isolated by magnetic cell sorting. The differential protein spots were analyzed by software analysis, subject to ingel digestion, and identified by matrix assistant laser desorption timeof - flight mass spectrometry （ MALDI - TOF - MS） and MALDI - TOF/TOF - MS. Results There were 73 differentially expressed protein spots observed in comparing between CD133 ＋ and CD133 - U87 cells by 2D - PAGE （ P 〈 0. 05）. Spots with 〉 1.5 - fold expression changes were 46, and 〉 2 - fold were 27, and 〉 3 - fold were 8. Ten spots were increased, while 63 spots were decreased in CD133 ＋ U87 cells. Forty - four high -quality peptide mass fingerprinting and 35 proteins were obtained by MS. Conclusions There are significant differences between CD133 ＋ and CD133- U87 cells. Differentially proteins have been obtained by proteomics analysis, which are important targets to the study of brain cancer stem ceils （BTSCs）.
出处
《中华神经外科杂志》
CSCD
北大核心
2012年第5期486-489,共4页
Chinese Journal of Neurosurgery
基金
江苏省自然科学基金（BK2005156）
江苏省卫生厅面上科研项目（H2006040）
镇江市重点医学人才项目志谢：衷心感谢“中国科学院上海生命科学研究院蛋白质组研究分析中心”在“蛋白质组学”研究中所提供的帮助.
