口服Ⅱ型胶原肽-霍乱毒素B亚单位-脂质体复合物诱导免疫耐受的研究

The research of tolerance induced by oral collagen type H peptide-cholera toxin B subunit-liposome complex

目的观察口服Ⅱ型胶原肽-霍乱毒素B亚单位-脂质体复合物对免疫耐受的诱导作用。方法DBA／1小鼠分组，Ⅰ组：正常对照组，Ⅱ组：胶原诱导关节炎对照组，Ⅲ组：口服Ⅱ型胶原肽-霍乱毒素B亚单位-脂质体复合物组，Ⅳ组：免疫后14d测定IgG2a组。记录分析关节炎评分及组织病理学评分，采用ELISA方法测定血清IgG2a水平。结果正常对照组小鼠无关节炎发生。Ⅱ组关节炎发生率显著高于Ⅲ组（100％vs28．6％，P〈0．05）；Ⅱ组关节炎评分显著高于Ⅲ组（5．40VS0．43，P〈0．01）；Ⅱ组关节炎组织病理累计评分显著高于Ⅲ组（16．00VS2．85，P〈0．05）。I组IgG2a水平极低，为38ng／ml，Ⅱ组IgG2a水平显著升高，达3922．g／ml，Ⅲ组IgG2a水平为3219ng／ml，较Ⅱ组降低（P〈0．05）；Ⅳ组IgG2a水平为98ng／ml，较Ⅰ组升高（P〈0．01）。结论口服Ⅱ型胶原肽一霍乱毒素B亚单位-脂质体复合物可诱导免疫耐受，缓解关节炎的进展。

Objective To investigate oral collagen type II peptide-cholera toxin B subunit-lipo- some complex induce tolerance to collagen-induced arthritis （CIA）. Methods DBA/1 mice were divided into four groups, group I ： normal control, group II ： CIA control, group HI ： oral collagen type II peptidecholera toxin B subunit-liposome complex, and group IV ： for testing IgG2a （ on day 14 after primary immunization）. Arthritis scores and histopathologic assessment were analyzed. The levels of serum IgG2a were examined by ELISA. Results There was no arthritis development in group I. The incidence of arthritis in group II was higher than that in group III （100% vs 28.6%, P〈0.05）. The arthritis score in group 1I was higher than that in group 11I （5.40 vs 0.43, P〈0.01 ）. Histopathologic score was higher in group 11 than that in group HI （ 16.00 vs 2.85, P〈0.05 ）. Level of serum IgG2a of group I was very low （38 ng/ml）. Mice of group 11 produced significantly higher level of IgG2a than mice of group HI （ 3922 ng/ml vs 3219 ng/ml, P〈0.05）. IgG2a of group Ⅳ was 98 ng/ml which was significantly higher than that of group I （P〈 0.01 ）. Conclusion Oral collagen type II peptide-cholera toxin B subunit-liposome complex could inhibit CIA progression through immune tolerance.