缺氧缺血型狭长窄蒂皮瓣成活过程中SDF-1表达的实验研究

Experiment study of SDF-1 expression during the survival process of the narrow pedicle flap with hypoxia and ischemia

目的检测基质细胞衍生因子-1（stromal cell derived factor 1，SDF-1）在缺氧缺血型狭长窄蒂皮瓣组织不同时间、不同面积的表达，研究SDF-1／CXCR4轴在狭长窄蒂皮瓣组织血管新生中的作用。方法在5头家猪双侧背部均制作蒂部长宽比例为4：2，携带面积分别为2cm×2cm（A组皮瓣）、3cm×3cm（B组皮瓣）、4cm×4cm（C组皮瓣）、5cm×5cm（D组皮瓣）和6cm×6cm（E组皮瓣）的狭长窄蒂皮瓣模型。对5组皮瓣进行大体观察、成活面积计算、皮瓣组织HE染色，用ELISA法检测皮瓣组织远端在术中及术后3、5、7、14d时SDF-1表达变化情况。结果①同一组狭长窄蒂皮瓣，随着时间推移，SDF-1的表达也随之增加，在第5天达到最高值，随后逐渐下降，趋于基础水平。②不同组狭长窄蒂皮瓣，随着皮瓣面积的增大，SDF-1的表达也随之增加，当皮瓣面积达5cm×5cm时，SDF-1的表达量不再增加，皮瓣远端部分坏死。结论SDF-1的表达量可能与狭长窄蒂皮瓣成活过程中的供血量相关。

Objective To detect the stromal cell derived factor 1 （SDF-1） expression during the survival process of the narrow pedicle flap with hypoxia and ischemia and to investigate the role of SDF-1/ CXCR4 axis in flap neovascularization. Methods The narrow pedicle flaps were formed on the bilateral back of 5 pigs. The pedicle ratio of length to width was 4： 2. The flap size was 2 cm × 2 cm（ group A） , 3 cm ×3 cm（group B）, 4 cm ×4 cm（group C）, 5 cm ×5 cm（group D）, 6 cm ×6 cm（group E）. The flaps survival rate was observed and HE staining was performed. The SDF-1 expression at the distal end of flaps was detected by ELISA during the operation and 3, 5, 7, 14 days after operation. Results （1） SDF-1 expression at the same group increased after operation until it reached the peak value at 5 days after operation; then it decreased to basic value. （2） SDF-1 expression in different groups was higher in bigger flaps until the flaps size reached 5 cm× 5 cm. Then partial necrosis happened at the distal end of flaps. Conclusions The SDF-1 expression may be related to the blood supply during the survival process of the narrow pedicle flap with hypoxia and ischemia.