乙二胺四乙酸联合正丁醇在大鼠胫骨癌痛标本石蜡切片制作中的应用

Combining ethylene diamine tetraacetic acid with N-butanol to prepare paraffin tissue sections from the rat with tibia bone cancer pain

目的探索一种简便有效的制作大鼠胫骨癌痛标本石蜡组织切片的方法。方法取大鼠胫骨癌痛模型组和正常组胫骨标本,使用乙二胺四乙酸(EDTA)加温脱钙联合正丁醇取代传统强酸脱钙、纯酒精脱水和二甲苯透明,制作石蜡切片后HE染色观察。结果两组大鼠胫骨癌痛标本形态结构均保存完整。与传统方法相比,制片步骤简单,脱水和透明时间较为灵活,蜡块切割性能较好。结论 EDTA与正丁醇的联合应用,是大鼠胫骨癌痛标本石蜡组织切片制作适宜和可靠的方法。

Objective To explore a simple and effective method for preparing paraffin tissue sections using specimens of tibia bone cancer pain in rats. Methods Specimens of tibia bone were obtained from the rats of bone cancer pain model group and normal group. The specimens were decalcified with the heated ethylene diamine tetraacetic acid （EDTA） , followed by the dehydration and clearing with N-butanol instead of traditional methods of decalcification in strong acid, dehydration through absolute alcohol and clearing in xylene. The specimens were stained with hematoxylin and eosin. Results The morphological structures of specimens in both groups were intactly preserved. Compared with the traditional method, the present method had the following advantages： the procedure was simple, the duration of dehydration and clearing was more flexible, and the paraffin blocks shown a better cutting performance. Conclusion It is a suitable and reliable method to jointly use EDTA and n-butanol in preparing paraffin tissue sections using specimens of tibial bone cancer pain in rats.