缺氧诱导因子1α和胰岛素在胰腺癌中的表达及相关性

Expression of hypoxia inducible factor-1a and insulin in pancreatic cancer and their correlation

目的 探讨缺氧诱导因子1a（HIF-1a）蛋白和胰岛素在胰腺癌组织中的表达及其相关性。方法采用免疫组织化学sP法检测65例胰腺癌中心癌组织和边缘癌组织中HIF-1a和胰岛素的表达情况；Westernblot法检测28例胰腺癌中心癌组织和边缘癌组织中HIF-1a蛋白和胰岛素的表达水平，并分析其相关性。结果HIF-1a蛋白在65例胰腺癌中心癌组织和边缘癌组织中的阳性表达率分别为70．8％和66．2％，差异无统计学意义（P〉0．05）。HIF—1a蛋白在28例胰腺癌中心癌组织和边缘癌组织中的表达量分别为0．338±0．030和0．327±0．035，差异无统计学意义（P〉0．05）。胰岛素在胰腺癌中心癌组织和边缘癌组织中的阳性表达率分别为41．5％和80．0％，差异有统计学意义（P〈0．05）。胰岛素在28例胰腺癌中心癌组织和边缘癌组织中的表达量分别为0．191±0．016和0．584±0．022，差异有统计学意义（P〈0．05）。相关性分析显示，胰腺癌边缘癌组织中HIF-1a蛋白与胰岛素水平有关（r=0．374，P〈0．05），呈明显正相关性（r=0．89，P〈0．05）；胰腺癌中心癌组织中HIF．10t蛋白与胰岛素水平无相关性（r=-0．145，P〉0．05）。结论通过调控HIF-1a蛋白的表达，胰岛素可增强胰腺癌细胞的侵袭与转移能力。

Objective To explore the correlation between the expressions of hypoxia inducible factor-let （HIF-let） and insulin in pancreatic cancer. Methods HIF-1a and insulin expression was detected by immunohistochemistry in the center and the edge of pancreatic adenocarcinoma specimens of 65 cases. Western blot was used to detect HIF-1a expression and insulin level in the center and the edge of pancreatic adenocareinoma specimens of 28 cases. The relationship between HIF-1a expression and insulin level in the pancreatic cancer was analyzed. Results The results of immunohistochemistry and Western blot showed that HIF-1a protein expression was high in both the center and the edge of pancreatic cancers （ P 〉 0.05） , and insulin level was significantly higher at the edge of specimen than that in the center （P 〈 0. 05 ）. HIF-1a protein and insulin levels were positively correlated at the edge of cancer tissue （r = 0. 374, P 〈 0.05 ） , but no significant correlation between them in the center of cancerous tissue （ r = - 0. 145, P 〉 0. 05）. Conclusion Insulin may promote the local invasion and metastasis of pancreatic cancer by upregulating HIF-1 a.