油菜BnMKK4全长基因的克隆及表达分析

Cloning and Expression Analysis of a BnMKK4 Gene from Brassica napus L.

从‘陇油6号’油菜中克隆得到了一个新的BnMKK4基因的cDNA,全长1317bp,其中包括993bp的开放阅读框,159bp的5'非翻译区(5'UTR),165bp的3'非翻译区(3'UTR)。与拟南芥AtMKK4有很高的同源性,因此命名为BnMKK4(GenBank登录号为JF268686)。该基因编码330个氨基酸的蛋白质,分子量36.5kDa,等电点为9.01。实时荧光定量PCR结果显示该基因表达受低温胁迫和盐胁迫的诱导,表明该基因在油菜适应低温胁迫和盐胁迫的过程中发挥作用。

In this study, we isolated a novel MAPKK gene, BnMKK4, from Brassica napus. The cloned fulllength cDNA was 1 317 bp, contained a 993-bp opening reading flame （ORF）, a 5＇-untranslated region （5＇ UTR） of 159 bp, and a 3＇-untranslated region （3＇ UTR） of 165 bp. The BnMKK4 exhibited closest homology to AtMKK4, so it was named the BnMKK4 （GenBank accession No. JF268686）. The deduced protein was 330 amino acids with molecular weight 36.5 kDa and isoelectric point 9.01. RT-PCR analysis revealed that the transcript level of BnMKK4 was higher in experimental plants than in control plants. The results suggested that the BnMKK4 gene is involved in response to cold and salt stresses and that BnMKK4 played an important role during cold and salt stresses in Brassica napus.