金龟子绿僵菌IMI330189液体发酵动力学研究

Kinetic study of liquid fermentation for Metarhizium anisopliae IMI330189

研究了金龟子绿僵菌IMI330189的液体发酵动力学。利用Sigmoid函数构建了该菌株液体发酵过程中的菌体生长和底物消耗的动力学模型,并运用Origin7.5软件拟合求解出各模型参数。结果表明,模型能够较好地拟合绿僵菌IMI330189液体发酵过程,其比生长速率在发酵第22.8h达到最大值,为0.084h-1;总糖比消耗速率在第9.6h达到最大值,为0.246h-1;总氮比消耗速率在第10.3h达到最大值,为0.007h-1;菌体对总糖的得率系数在39.8h达到最高,为0.861g/g。模型拟合和实验数据具有良好的适应性,基本反映了绿僵菌IMI330189液体发酵过程的动力学特征,为其液体发酵工艺的优化和发展奠定了基础。

The liquid fermentation kinetics ofMetarhizium anisopliae IMI330189 was observed. Kinetic models of biomass growth and substrate consumption were established based on Sigmoid model and the parameters of the models were determined by Origin7.5 software. The results showed that the models fitted well with the liquid fermentation process of M. anisopliae IMI330189. The maximum specific growth rate was found to be 0.084h^-1 at 22.8h, specific total sugar consumption rate 0.246h^-1 at 9.6h and specific total nitrogen consumption rate 0.007h^-1 at 10.3h. The maximum yieldcoefficient of biomass from total sugar was 0.861g/g at 39.8h. The model simulation matched well with the experimental observations, which made it possible to elucidate the kinetic characteristics of M. anisopBae IMI330189 during liquid fermentation. These models thus can be employed for the development and optimization of M. anisopliae IMI330189 liquid fermentation processes.