IL-23促进人肺腺癌A549细胞的迁移和侵袭

Interleukin 23 Promotes Lung Adenocarcinoma A549 Cell Migration and Invasion

背景与目的前炎症因子白细胞介素23(interleukin 23,IL-23)是与慢性炎症和肿瘤微环境相关的一种重要的细胞因子,同时IL-23受体在结直肠癌、肺癌、口腔鳞癌等肿瘤细胞中有表达。本研究旨在探讨IL-23能否促进人肺腺癌细胞A549的迁移和侵袭并探讨其机制。方法用划痕试验和Transwell小室法测定IL-23对A549的迁移和侵袭的影响,用Real-time PCR和ELISA检测IL-23对基质金属蛋白酶9(matrix metalloproteinase 9,MMP-9)的mRNA和蛋白表达的影响,通过IL-23中和抗体阻断IL-23的作用,进一步证实IL-23对A549迁移和侵袭的影响。结果 IL-23明显增加了A549细胞的迁移和侵袭能力;同时IL-23能提高A549细胞MMP-9的mRNA表达和其培养上清中MMP-9的蛋白表达,IL-23中和抗体能有效地阻断IL-23对A549的迁移和侵袭的作用。结论 IL-23可刺激A549细胞表达MMP-9,从而促进A549细胞的迁移和侵袭。

Background and objective Interleukin 23（IL-23） is a pro-inflammatory cytokine that plays an important role in inflammatory disease and tumor microenvironment.The IL-23 receptor is expressed in colon,lung,and oral carcinomas.We performed this study to investigate whether IL-23 promotes directly carcinoma cell migration and invasion as well as further explore its mechanism.Methods The migration and invasion of human lung adenocarcinoma A549 cells induced by IL-23 were detected by a scratch test and Transwell experiment.MMP-9 expression of the mRNA and protein levels of A549 cells cultured with and without IL-23 was respectively detected by Real-time PCR and ELISA.The effect of IL-23 on A549 cells was further verified using anti-IL-23p19 neutralization antibody（Ab IL-23p19） to eliminate IL-23.Results IL-23 remarkably promoted A549 cell migration and invasion.MMP-9 expression in A549 cells was upregulated by IL-23 stimulation.In addition,the effect of IL-23 on the migration and invasion of A549,as well as the MMP-9 expression in A549 cells induced by IL-23,was eliminated by Ab IL-23p19.Conclusion IL-23 promotes the migration and invasion of A549 cells by inducing MMP-9 expression.