健脾消胀片对阿托品致小鼠胃肠蠕动降低模型肠蠕动的影响

Effect of Jianpi Xiaozhang Pian on the enterokinesia of the mice with depressed gastrointestinal peristalsis induced by atropine

目的观察健脾消胀片对阿托品致小鼠胃肠蠕动降低模型肠蠕动的影响。方法 70只小鼠随机分为7组,每组10只,其中6组腹腔注射0.1 mg.kg-1阿托品制作小鼠胃肠蠕动降低模型,另1组不做处理为空白对照组。根据灌服混悬液情况将6组分为大、中、小剂量健脾消胀片组、多潘立酮组、保和丸组和模型组,大、中、小剂量健脾消胀片组分别灌服大(180 g.L-1)、中(90 g.L-1)、小(45 g.L-1)剂量健脾消胀片混悬液;多潘立酮组灌服多潘立酮片混悬液;保和丸组灌服保和丸混悬液。模型组、空白对照组灌服同体积羧甲基纤维素钠。1 h后,各组均灌服炭末混悬液。计算各组炭末推进率,观察各组小鼠首次出现含炭末粪便的潜伏期及6 h出现含炭末粪便的粒数。结果与空白对照组比较,模型组首次出现含炭末粪便的潜伏期显著延长(P<0.01),6 h出现含炭末粪便的总数明显减少(P<0.01);与模型组比较,大、中剂量健脾消胀片组、多潘立酮组和保和丸组均可显著缩短模型小鼠首次出现含炭末粪便的潜伏期(P<0.01)。与空白对照组比较,模型组炭末推进率显著降低(P<0.01);与模型组比较,大、中剂量健脾消胀片组、多潘立酮组和保和丸组炭末推进率均显著提高,差异均有统计学意义(P<0.05)。结论健脾消胀片对阿托品致小鼠胃肠蠕动降低模型具有促进其肠蠕动作用。

Objective To explore the effect of Jianpi Xiaozhang Pian on the enterokinesia of mice with depressed gastrointestinal peristalsis induced by atropine.Methods Seventy mice were randomly divided into seven groups：large,medium and small doses of Jianpi Xiaozhang Pian groups,domperidone group,Baohewan group and model group,ten mice in each group.Among the groups,the mice in six groups were made as the model of depressed gastrointestinal peristalsis induced by 0.1 mg·kg-1 atropine through intraperitoneal injection,while in another group as blank control group.Then,the mice in the large,medium and small doses of Jianpi Xiaozhang Pian groups were respectively given with large（180 g·L^-1）,medium（90 g·L^-1） and small（45 g·L^-1） doses of Jianpi Xiaozhang Pian suspension by intragastric administration;the mice in domperidone group were given with domperidone suspension;the mice in Baohewan group were given with Baohewan suspension;the mice in model group and blank control group were given with the same volume of sodium carboxymethyl cellulose.All rats were given with carbon powder suspl by intraperitoneal injection after one hour.The rates carbon powder impelling of all groups were calculated,and the incubation period of carbon appeared in feces and the number of feces containing carbon after six hours.Results Compared with the blank control group,the incubation period of carbon appeared in feces in model group was were significantly prolonged（P〈0.01）,the number of feces containing carbon decreased obviously after six hours（P〈0.01）.Compared with the model group,the incubation period of carbon appeared in feces in the large and medium doses of Jianpi Xiaozhang Pian groups,domperidone group and Baohewan group was significantly shorter（P〈0.01）.Compared with the blank control group,the rate of carbon powder impelling in model group were significantly lower（P〈0.01）.Compared with the model group,the rates of carbon powder impelling were significantly higher in the large and medium doses of Jianpi Xiaozhang Pian group,domperidone group and Baohewan group（P〈0.05）.Conclusion Jianpi Xiaozhang Pian can promote the enterokinesia of mice with depressed gastrointestinal peristalsis induced by atropine.