内热针对大鼠软组织慢性损伤的影响

Effect of interior heat needle therapy on chronic soft tissues injury in rats

目的比较内热针疗法对大鼠腓肠肌肌肉肌腱处慢性损伤后形态学变化和脑源性神经营养因子(BDNF)水平的影响。方法 27只雄性Wistar大鼠随机分为损伤对照组(12只)、内热针组(12只)、正常对照组(3只)。正常对照组不做任何处理,损伤对照组和内热针组大鼠造模形成慢性软组织损伤,内热针组在治疗后3、7、14、28d,分别处死实验大鼠3只,损伤对照组不做治疗,自然饲养,按以上时间点分别处死大鼠3只,取双侧损伤组织,对组织进行光镜下形态学观察和BDNF免疫组化测定。结果内热针组形态学变化提示组织恢复情况较损伤对照组好,治疗3d后BDNF在内热针组与损伤对照组间无统计学差异(P>0.05),7、14d内热针组明显高于损伤对照组(P<0.05),28d内热针组低于损伤对照组(P<0.05)。结论内热针治疗可以显著提高BFGF含量,对大鼠软组织慢性损伤具有一定疗效。

Objective To compare the effect of interior heat needle therapy on morphology of chronic gastrocnemius muscle tendon injury and brain-derived neurotrophic factor（BDNF） level in rats.Methods Twenty-seven healthy male Wistar rats were randomly divided into injury control group（n=12）,interior heat needle therapy group（n=12） and normal control group（n=3）.Rat in normal control group and injury control group did not receive any treatment.A chronic soft tissue injury model was established.Three rats in injury control group and interior heat needle therapy group were killed 3,7,14 and 28d after treatment.Morphology of inured tissue and BDNF level were observed under optical microscope with immunohistological staining.Results The changes of morphology indicated that the tissue repair was better in interior heat needle therapy group than in injury control group.No significant difference was observed in tissue repair between the two groups 3d after treatment（P0.05）.However,the tissue repair was much better in interior heat needle therapy group than in injury control group 7 and 14d after treatment（P0.05） and less in interior heat needle therapy group than in injury control group 28d after treatment（P0.05）.Conclusion Interior heat needle therapy can significantly increase the basic fibroblast growth factor（BFGF） level and is thus effective for chronic soft tissue injury in rats.