慢性萎缩性胃炎的动物模型研究

Establishment of animal chronic atrophic gastritis model

目的通过多因素刺激大鼠胃黏膜,建立稳定的慢性萎缩性胃炎的大鼠模型并初步探讨造模方法对于肝脏的影响。方法将大鼠随机分为模型组与正常组,模型组自由饮用0.1%氨水,前10周用2%水杨酸钠和30%酒精混合溶液灌胃,后12周用20mmol/L去氧胆酸钠溶液灌胃;正常对照组正常饮水,用饮用水灌胃22周。结果与正常组比较,模型组大鼠一般情况较差,体重增加缓慢,胃窦腺体萎缩明显,黏膜层变薄且黏膜肌层相对增厚,炎性细胞浸润明显;两组均未见到肠化生及异型增生等现象。两组肝脏病理均无明显异常。结论利用多因素刺激成功建立了萎缩性胃炎模型,且此模型对肝脏病理影响不明显。

Objective To establish an stable experimental model of chronic atrophic gastritis（CAG） in rats and investigate the effect on the liver preliminarily by stimulating rat gastric mucosa with multiple factors.Methods Rats were randomly divided into model group and normal control group.Rat in model group had free access to 0.1% ammonia water and drank a mixture of 2% sodium salicylate and 30% alcohol for the first 10 weeks followed by 20mmol/L sodium deoxycholate for the next 12 weeks,while those in normal group had free access to drinking water for 22 weeks.Results The general condition was worse,the body weight gain was slower,the atrophy of glands was more significant,the mucosa was thinner,the muscular layer of mucosa was thicker,and the infiltration of inflammatory cells was severer in model group than in normal control group.No intestinal metaplasia and dysplasia,and no obvious abnormality of liver pathology were observed in two groups.Conclusion A CAG model is successfully established by stimulating gastric mucosa with multiple factors,with no obvious effect on liver pathology.