摘要
目的观察微波辐射对大鼠学习记忆能力和海马氨基酸类神经递质含量的影响。方法采用30 mW/cm2微波辐射56只Wistar雄性大鼠,于辐射后6 h、1 d、7 d、14 d和28 d,采用Morris水迷宫试验的平均逃避潜伏期检测大鼠的学习和记忆能力的改变;并于辐射后1 d、7 d、14 d和28 d,采用高效液相色谱法(HPLC)大鼠海马组织中天冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)及γ-氨基丁酸(GABA)4种氨基酸类神经递质含量的变化。结果微波辐射后6 h和7 d,大鼠的平均逃避潜伏期延长,与假辐射组相比有显著差异(P<0.05);辐射后1 d,辐射组Asp、Glu与GABA含量降低,与假辐射组相比有显著差异(P<0.01);辐射后7 d,辐射组Asp和Gly含量升高,与假辐射组相比有显著差异(P<0.05)。结论微波辐射可造成大鼠空间学习记忆能力下降及海马氨基酸类神经递质含量的改变,并且具有可恢复性。
Objective To investigate the effects of microwave radiation on the learning and memory ability and amino acid neurotransmitter levels hippocampus of rats. Methods Fifty- six male Wistar rats were exposed to microwave radiation with an average power dens two days for 3 times. The learning and memory ability was assessed Morris water maze test at 6 h, 1 d, 7 d, 14 and GABA were measured by HPLC at 1 d, escape latency was significantly longer at 6 h lty by of 30 mW/cm2 for 10 min, every the average escape latency in the d and 28 d after radiation. The contents of Asp, Glu, Gly 7 d, 14 d and 28 d after irradiation. Results The average and 7 d after exposure to 30 mW/cm2 microwave radiation,compared with that in the controls （P 〈 0. 05）. The contents of Asp, Glu and GABA in the hippocampus were significantly decreased at ld after irradiation （P 〈0. 01 ）, and the contents of Asp and Gly in the hippocampus were significantly increased at 7 d after irradiation （ P 〈 0. 05 ）. Conclusions 30 mW/cm^2 microwave radiation can induce the decline of the learning and memory ability of space and changes of neurotransmitter contents in the hippocampus of rats which can be recoverable.
出处
《中国体视学与图像分析》
2012年第1期61-64,共4页
Chinese Journal of Stereology and Image Analysis
基金
国家自然科学基金(81172620)
