摘要
目的探讨类胰蛋白酶(tryptase)与冠心病病情及与冠状动脉病变程度的关系。方法从本教研室2008～2011年尸检档案中挑选病例及心脏标本共70例。分为四组:A组:冠心病猝死(SCD,20例);B组:冠心病非猝死者(CHD,20例);C组:过敏性猝死(10例);D组:阴性对照(无明显动脉粥样硬化病变的死者,20例)。应用免疫组化染色(SP法)和图像定量分析法,观察每例左心室缺血心肌的类胰蛋白酶染色情况和光密度。结果 SCD组、CHD组、过敏性猝死组光密度值均高于阴性对照组,各组间比较均有显著差异(P<0.01)。结论缺血心肌中类胰蛋白酶的表达增加与SCD的发生密切相关,为SCD的诊断和临床对冠心病防治的研究提供新的途径。
Objective To investigate therelationship between tryptase expression in patients ~vith coro- nary heart disease and severity of the coronary artery disease. Methods Autopsy heart samples （n = 70） collected during 2008-2011 were divided into 4 groups. Group A （20 cases） died of sudden death with coronary heart disease （SCD） ;group B （20 cases） ,non-sudden death with coronary heart disease;group C （10 cases） died of anaphylaxis sudden death; group D （20 cases）, died of any cause without atherosclerosis. Tryptase was detected by immunohistochemistry （ SP method） and image analysis system. Resuits OD in ischemic myocardium were significantly higher in SCD, CHD, sudden death of hypersensitivity up than that in the control group, and comparison among the groups showed statistically significantly differences （P 〈 0.05）. Condusions Tryptase expression is increased in ischemic myocardium of SCD patients. Increased expression： of tryptase in ischemic myocardium is closely related to the occurrence ofSCD, providing new ways for SCD diagnosis and clinical research of prevention and treatment of coronary heart disease.
出处
《中国体视学与图像分析》
2012年第1期75-80,共6页
Chinese Journal of Stereology and Image Analysis
基金
上海市法医学重点实验室开放课题(KF0906)
关键词
冠心病
尸检心脏
类胰蛋白酶
免疫组织化学
图像分析
coronary atherosclerosis
autopsy heart
tryptase
immunohistochemistry
image analysis
