血红素对人肾小管上皮HK-2细胞的损伤作用研究

Damaging effects of hemin on the human renal tubular epithelial cell line HK-2 cells

目的观察血红素对人肾小管上皮细胞HK-2的损伤作用。方法体外培养人肾小管上皮HK-2细胞,经50、100、150、200μmol/L血红素处理3、6、12 h后,MTT法检测细胞活性,光镜、电镜观察细胞病理形态改变。结果与对照组比较,50μmol/L血红素组细胞活性未见明显变化,100、150、200μmol/L血红素明显降低HK-2细胞活性;光镜下可见细胞皱缩、变圆;超微结构改变主要为微绒毛数量减少、内质网扩张和空化等。结论一定浓度的血红素降低HK-2细胞活性,引起其形态学改变。

Objective To investigate the damaging effects of hemin on HK-2 cells, a human renal tubular epithelial cell line. Methods HK-2 cells were cultured and treated with 50, 100, 150, 200μmol/L hemin for 3, 6, 12 h. Cell viability was measured by MTT assay, cell morphology was examined by phase contrast microscopy and electron microscopy. Results 50 μmol/L hemin had no significant effect on the cell viability, 100, 150, 200 μmol/L hemin markedly resulted in a decrease in cell viability when compared with the control group. Hemin caused cell shrinkage and rounding, a reduced number of mirovilli, and dilation and vacuolization of endoplasmic reticulumin in the HK-2 ceils. Conclusion Hemin causes a decrease in cell viability and remarkable morphological changes of HK-2 cells.