曲霉菌预处理前后大鼠角膜上皮TLR4受体的表达

Expression of TLR4 in rats' corneal epithelium with pretreatment of Aspergillus

目的:检测曲霉菌预处理前后大鼠角膜上皮TLR4受体的表达变化。方法:健康Wistar大鼠24只随机分为A、B、C、D共4组,A组不予特殊处理,B、C、D三组以罩杯法刺激大鼠角膜4h建立曲霉菌刺激模型。B组在完成刺激后立即取角膜上皮,C组在96h后取角膜上皮,D组在96h后重复刺激,其后取角膜上皮,采用Real-timePCR法检测所取上皮组织中TLR4受体mRNA的表达。结果:罩杯法建立大鼠角膜曲霉菌刺激模型后TLR4受体mRNA的表达明显上升,但96h后已趋于正常,再次刺激后受体mRNA的表达变化与初次刺激差异无统计学意义(P>0.05)。结论:罩杯刺激法可以使TLR4受体mRNA表达上升,但受体并未因曲霉菌刺激而产生基础表达量的变化,重复刺激后受体表达亦无明显差异。

AIM： To detect the expression of TLR4 in rats＇ corneal epithelium with pretreatment of Aspergillus. METHODS： Healthy Wistar rats were randomly divided into four groups of A, B, C and D. Group A was the blank control group. Rats＇ corneal epithelium was stimulated by Aspergillus for 4 hours in group B,C and D. Rats＇ corneal epithelium was scraped immediately in the completion of the stimulation in group B. The epithelium was scraped after 96 hours in group C. The epithelium was stimulated again by Aspergillus after 96 hours in group D, and was scraped immediately. Real-time PCR was used to assay the mRNA expression of TLR4 receptor in the epithelial tissue. RESULTS： The expression of TLR4 was significantly increased by the stimulation of Aspergillus, and it became normal after 96 hours. The expression of TLR4 was no significant difference after twice stimulations. CONCLUSION： The expression of TLR4 can be increased by the stimulation of Aspergillus, but the basic expression level has no significant change.