Th17细胞相关因子在人脑胶质瘤中的测定

The detection of Th17 cell correlation factors expression in glioma

目的了解Th17细胞相关因子在人脑胶质瘤组织中的表达,确定Th17的存在及浸润程度。方法应用免疫组化二步法检测Th17细胞相关因子CD4+和IL-17在34例脑胶质瘤和10例正常脑组织病理切片中的表达,采用实时荧光定量逆转录聚合酶链反应(QRT-PCR)技术,检测IL-17mRNA、RORγtmRNA在人脑胶质瘤组织中的表达量。结果 IL-17、CD4+在正常脑组织表达不明显,在低级别脑胶质瘤中表达较少,CD4+和IL-17在高级别脑胶质瘤中表达明显,组间比较差异有统计学意义(P<0.05,P<0.05,P<0.01)。IL-17mRNA和RORγt mRNA在正常脑组织、低级别、高级别脑胶质瘤中的表达量随恶性程度升高而增加,组间比较差异有统计学意义(P<0.05,P<0.01,P<0.01)。结论脑胶质瘤中存在Th17的浸润,其表达量随脑胶质瘤恶性程度的升高而增加。

Objective To investigate Th17 existence and infiltration in glioma by testing Th17 correlation factors ex- pression. Methods The presence of CD4 ＋ and IL-17 were measured by immunohistochemistry in 34 human glioma tissues and 10 normal brain tissues. Expression levels of both IL-17mRNA and RORγtmRNA were detected by QRT- PCR technology. Results Neither CD4 ＋ nor IL-17 had positive staining in the 10 normal brain tissues. In the low grade group （WHO Ⅰ- Ⅱ ）, there were low expression levels of both CD4 ＋ and IL-17. Compared with those of the low-grade group, there were obviously higher expression levels of both CD4 ＋ and IL-17 in the high-grade group （WHO HI- 1V ）. There was significantly statistical difference among the comparisons （ P 〈 0. 05, P 〈 0. 05, P 〈 0.01 ）. The QRT-PCR result showed that expression levels of IL-17AmRNA and RORγtmRNA had a positive correlation with the malignancy of human glioma. There was significant statistical difference among the comparisons of the three groups （P 〈 0. 05 ,P 〈 0. 05 ,P 〈 0.01 ）. Conclusion There is Thl7 infiltration in glioma, whose expression level has a posi- tive correlation with the malignancy of human glioma.