黄芪注射液对脑缺血/再灌注大鼠海马神经元Caspase-3表达的影响

Effect of astragalus injection on the expression of Caspase-3 after cerebral ischemia and reperfusion in hippocampal neurons of rats

目的观察黄芪注射液对脑缺血/再灌注大鼠海马神经元Caspase-3表达的影响。方法将132只♂SD大鼠随机分为4组:假手术组、模型组、黄芪注射液溶剂对照组及黄芪注射液组。采用四血管阻断法建立大鼠脑缺血/再灌注模型[1],于再灌注后0、0.5、2、6、24、72和120 h断头取脑。采用免疫组织化学法、Western blot法检测大鼠海马组织Caspase-3蛋白的表达,实时荧光PCR法检测Caspase-3 mR-NA的表达。结果模型组除0和0.5 h外,其余各个时间点Caspase-3蛋白及mRNA表达均较假手术组增加(P<0.05);与模型组相比,黄芪注射液组除0和0.5 h外其余各个时间点Caspase-3蛋白及mRNA表达均减少(P<0.05),而黄芪注射液溶剂对照组则无明显变化(P>0.05)。结论黄芪注射液能抑制大鼠海马神经元Caspase-3的表达,从而抑制海马神经元的凋亡,保护神经元。

Aim To investigate the effect of astragalus injection on the expression of Caspase-3 after global cerebral ischemia-reperfusion in hippocampal neurons of rats.Methods The 132 male SD rats were randomly divided into 4 groups,sham-operated group,model group,original astragalus injection group and astragalus injection group.The global cerebal ischemia-reperfusion model of rats was established by 4 vessel occlusion.After reperfusion 0 h,0.5 h,2 h,6 h,24 h,72 h and 120 h brains were removed,using immunohistochemistry and Western blot to detect hippocampus neurons Caspase-3 protein expression.The expression of Caspase-3 mRNA was observed by Real-time PCR.Results Compared with sham-operation group,the expression of Caspase-3 protein and mRNA at every time point increased obviously in model group except 0h and 0.5 h（P0.05）.Compared to model group,the expression of Caspase-3 protein and mRNA at every time point decreased obviously in astragalus injection group except oh and 0.5 h（P0.05）.However,the original astragalus injection group had no obvious change（P0.05）.Conclusion Astragalus injection can inhibit the expression of Caspase-3 after global cerebal ischemia-reperfusion,and accordingly inhibit apoptosis in hippocampal neurons,playing an important role in neuron protection.