涎腺腺样囊性癌中ADAM-17的表达及临床意义

The clinicopathological significance of ADAM-17 expression in salivary adenoid cystic carcinoma

目的探讨ADAM-17在涎腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC)中的表达及临床意义。方法收集48例SACC石蜡组织和20例新鲜组织,分别采用免疫组化EnVision两步法和RT-PCR法检测SACC组织中ADAM-17蛋白和mRNA表达水平,并分析其与临床病理学参数之间的相关性。结果 ADAM-17蛋白在48例SACC组织中阳性率为72.9%(35/48),ADAM-17mRNA水平在20例SACC中平均相对表达量为(0.40±0.18),两者的表达均高于正常对照组,差异具有统计学意义(P<0.001),两者的表达与肿瘤的大小、TNM分期和患者的生存时间均有相关性。结论 ADAM-17在SACC中高表达,可能介导癌组织生长、侵袭和转移,其有望成为一个新的治疗靶点。

Purpose To investigate the expression of ADAM-17 in salivary adenoid cystic carcinoma and to explore its correlation with clinical behavior and pathological characters. Methods 48 cases of the paraffin-embedded tissues and 20 cases of fresh tissues of SACC were collected to detect the ADAM-17 protein by immunohistochemistry and mRNA expression levels by RT-PCR. We also ana- lyzed the correlation between the expression of ADAM-17 and the clinical pathological parameters. Results The expression of ADAM- 17 protein was 72. 9% （35/48） in the 48 cases, and the average relative expression level of ADAM-17 mRNA levels was 0. 40 _＋0. 18 in the 20 cases, which were higher than that in the normal control group （ P 〈 0. 001 ）. And both were relation with the tumor size, TblM stage and the patient survival time. Conclusion ADAM17 is overexpressed in SACC and possibly mediate the cancer growth, invasion and metastasis, which is expected to become a new therapeutic target.