草鱼ATGL基因的表达及饲喂n-3 HUFAs对其的影响

ATGL gene expression and the influence of n-3 HUFAs in grass carp(Ctenopharyngodon idellus)

实验获得了草鱼脂肪组织甘油三酯水解酶（ATGL）部分cDNA序列（GenBank登录号为HQ845211）,并进行了序列同源性分析;采用实时定量反转录聚合酶链式反应（qRT-PCR）方法,检测了ATGL基因在草鱼不同组织的表达状况;研究了投喂n-3高不饱和脂肪酸（n-3 HUFAs）对草鱼肝胰脏ATGL基因时序表达的影响。结果显示,所获得的草鱼ATGL基因部分cDNA序列长度为687 bp,与人、牛、小鼠、长腭泥虎鱼、大黄鱼等物种的同源性为65%~75%;该基因在草鱼心脏、肝胰脏、脾脏、鳃、肾脏、肌肉、腹腔脂肪组织、脑、小肠、精巢10个组织中均有表达,其中在腹腔脂肪组织中表达丰度最高,在肝胰脏和肌肉中表达丰度次之。处理组草鱼摄食n-3 HUFAs饲料后,ATGL基因的表达水平在第1周和第2周显著高于对照组,第3周后,该基因的表达水平在处理组与对照组间无显著差异。研究首次克隆得到草鱼ATGL基因部分cDNA序列,并发现该基因在草鱼脂质蓄积及代谢较旺盛的组织中表达水平较高,且其在肝胰脏中的表达受到n-3 HUFAs的影响,其规律为先被诱导升高,然后回复到正常水平。

The partial cDNA of adipose triglyceride lipase（ATGL） was cloned（GenBank accession num-ber HQ845211）,and the homologous alignment was analyzed in grass carp Ctenopharyngodon idellus.The expression of ATGL gene in ten different organs was detected with the methods of qRT-PCR.The influ-ence of dietary n-3 highly unsaturated fatty acids（n-3 HUFAs） on its temporal expression in hepatopan-creas of grass carp was also studied.The results showed that the obtained cDNA sequence is 687bp in length,and its homology with human,cow,mice,large yellow croaker,long-jawed mudsucker and other species is 65% 75%.It is revealed that ATGL is widely expressed in heart,hepatopancreas,spleen,gills,kidney,muscle,intrapaneal fat body（IPF）,brain,intestine,spermary,with the highest expression level observed in IPF.The expression level of ATGL gene in the feeding trial was increased significantly during the first and second weeks,and then appeared to have no difference compared with that of control group.Our results indicate that ATGL is mainly expressed in lipid highly metabolized and deposited tissues in grass carp,and dietary n-3 HUFAs influence the expression of ATGL gene in hepatopancreas,with the expression pattern described as rising at first,then going down to the normal level.