PPARγ和糖皮质激素受体对肝细胞糖代谢的影响

Effect of PPARΥand glucocorticoid receptor on hepatic glucose metabolism

以地塞米松、糖皮质激素受体（GR）阻断剂RU486和吡格列酮孵育HepG2细胞24或48h，测定培养液中的葡萄糖浓度。结果显示，48h时10μmol／LRU486阻断地塞米松的升糖作用，但RU486＋吡格列酮组较吡格列酮组培养液中葡萄糖浓度升高（P〈0．05），提示PPARΥ和GR在肝细胞糖代谢方面可能存在相互作用。

HepG2 cells were incubated with dexamethasone, glucocorticoid receptor （GR） antagonist RU486, and pioglitazone for 24 or 48 h. Glucose concentration in culture medium was detected. The results showed that 10 μmol/L Rug86 blocked the surge of glucose concentration by 48 h of dexamethasone treatment and the glucose concentration in RU486 ＋ pioglitazone group was higher than pioglitazone group（ P〈0.05 ）, suggesting that the cross talk between PPARΥ, and GR may exist in hepatic glucose metabolism.