牙髓干细胞分化过程中L型钙离子通道羧基末端的表达

Expression of L-type calcium channel C terminus during dental pulp stem cell differentiation

目的:探讨牙髓干细胞(DPSCs)分化过程中L型钙离子通道羧基末端的表达。方法:利用酶消化法体外分离、培养大鼠牙髓干细胞;吉姆萨染色法检测大鼠牙髓干细胞的克隆形成能力;神经诱导体系下诱导牙髓干细胞向神经样细胞分化,免疫荧光染色检测细胞分化后胶质纤维酸蛋白(glial fibrillary acidic pro-tein,GFAP)的表达和细胞分化前后L型钙离子通道Cav 1.2及羧基末端的表达。结果:牙髓干细胞的克隆形成能力为每1 000个细胞形成2～17个克隆;免疫荧光染色检测诱导后细胞GFAP表达阳性;免疫荧光染色检测显示:牙髓干细胞分化前L型钙离子通道Cav 1.2羧基末端表达于细胞膜上,细胞分化后羧基末端同时表达于细胞膜上和细胞核中。结论:L型钙离子通道Cav 1.2羧基末端在牙髓干细胞分化过程中发生核转位,羧基末端可能在牙髓干细胞的分化过程中发挥着一定的作用。

AIM： To identify the expression of L-type calcium channel C terminus during dental pulp stem cells （DPSCs） differentiation. METHODS： Dental pulp stem cells were acquired by enzymatic digestion and cultured in vitro. The colony-forming efficiency of DPSCs was detected by Giemsa staining. DPSCs were induced to differentiate into neural-like cells. GFAP was detected by immunofluorescence staining after DPSCs differentiation. Expression of Cavl. 2 and C terminus was analyzed by immunofluorescence staining before and after DPSCs differentiation. RESULTS：The colony-forming efficiency of DPSCs was 2-17 colones/10z. The cells were stained positive by GFAP after being induced by neural induction medium. The L-type calcium channel Cavl. 2 C terminus of undifferentiated DPSCs was only expressed on cell membrane, and the C terminus was located both on cell membrane and nucleus after DPSCs differentiated into neural-like cells. CONCLUSION： The L-ype calcium channel that C terminus translocated to the nucleus during the differentiation of dental pulp stem cells, indicateing that C terminus may take part in the dif- ferentiation process of dental ,ulD stem cells.