珍稀濒危植物金毛狗的SRAP分析

Analysis of Genetic Diversity of the Rare and Endangered Species Cibotium barometz by SRAP Markers

利用SRAP分子标记技术,对7个居群79株金毛狗进行遗传多样性分析。结果表明:10对SRAP引物组合共得到107条扩增条带,多态性条带比率为85.98%,Nei’s基因多样性指数为0.229 6,Shannon’s多样性指数为0.358 6,表明金毛狗居群水平具有较高的遗传多样性;金毛狗7个居群的总基因多样度为0.229 6,居群内遗传多样度为0.135 4,居群间的遗传分化指数为0.410 6,表明有41.06%的变异存在于居群间,有58.94%的变异存在于居群内;居群间基因流为0.717 8,表明居群间基因交流频率较低;遗传一致度和UPGMA聚类分析结果显示,生境条件相似的居群优先聚集,说明金毛狗种质亲缘关系与地理分布相关性不显著。

SRAP molecular markers were used to study the genetic diversity on population and individual levels of 79 Cibotium barometz which were from 7 populations. The results showed that： 10 SRAP primer combination amplified 107 bands with 91 （85.98%） polymorphic. Nei＇s gene diversity index of SRAP was 0. 229 6. Shannon＇s information index was 0. 358 6. That implied that there was high genetic diversity a- mong the popolations. The total genetic diversity was 0. 229 6. The genetic diversity within population and the coefficient of gene differentiation were 0. 135 4 and 0. 410 6, respectively. That implied that it was 41.06% of genetic diversity among populations,and was 58.94% of genetic diversity within populations. Gene flow among population was 0. 717 8 which implied that the gene flow was at a low level. The genetic similarity coefficient and the UPGMA dendrogram indicated that populations with similar conditions were clustered firstly, the geographical distribution was not distinctive.