肺结核病患者外周血T淋巴细胞上PD-1的表达及意义

Expression of PD-1 on T lymphocyes in peripheral blood of patients with tuberculosis pulmonary disease

目的研究程序性死亡因子-1(PD-1)在肺结核病患者外周血T淋巴细胞上的表达,初步探讨其在结核慢性发生发展中的意义。方法选择肺结核患者(30例)为研究对象。对照组40例,分别为20例确诊为肺部感染的患者及20例正常健康体检者。分别用流式细胞术及RT-PCR检测PD-1蛋白及mRNA在T淋巴细胞上的表达情况。采用ELISA法检测各组血清中肿瘤坏死因子α(TNF-α)、γ-干扰素(IFN-γ)细胞因子浓度。结果与两对照组相比,肺结核患者外周血T淋巴细胞上PD-1蛋白及mRNA的表达均明显升高(P<0.01),外周血TNF-α和IFN-γ水平明显降低(P<0.05)。肺部感染与健康体检者相比,各项指标均无统计学差异。结论 PD-1在肺结核病患者T淋巴细胞上的表达明显升高,这可能在肺结核病的发生、发展及慢性化过程中起重要作用。

This study aims to investigate the expression of programmed death-1 （PD-1） on peripheral blood T cells （PBTCs） in patients with tuberculosis pulmonary disease and the role of this expression in genesis and development of chronic tuberculosis pulmonary. Total of 30 patients with tuberculosis pulmonary disease, 20 patients with pulmonary infection, and 20 healthy adults were recruited in this study. The expressions of PD-1 protein and mRNA in PBTCs were detected by direct flow cytometry and half-quantitative RT-PCR, respectively. Serum levels of cytokines TNF-α and INF-γ were detected with ELISA method as well. Compared with two control groups, the expression level of PD-1 was markedly higher in the group with tuberculosis pulmonary disease （P 〈 0.01）, but the levels of TNF-α and IFN-γ in the supernatant were significantly lower. From the results, we presume that high PD-1 expression may play a role in the pathogenesis and development of chronic tuberculosis pulmonary.