前列腺特异性膜抗原新型剪接变异体PSME基因及蛋白的生物信息学分析

Bioinformatic analysis for a new alternatively spliced variant of prostate-specific membrane antigen

目的通过对PSME的生物信息学分析,更多的了解该基因及蛋白结构与功能的相关信息。方法运用生物信息学方法分析PSME基因结构,序列及其编码蛋白的理化性质和结构与功能特征,蛋白相互作用网络以及抗原表位。结果用ExPASy的Computer PI/Mw、SMART等软件对其氨基酸序列进行分析,该蛋白的PI值为6.38,相对分子质量约为78 000。二级结构中α螺旋(H)占34.66%,β折叠(E)占12.93%,无规卷曲占52.41%。PSME信号肽位于1～22位氨基酸,150～248位为肽酶结构域,639～703位为转铁蛋白受体二聚体,且含有多个糖基化位点。通过DNA Star软件分析得到了PSME蛋白的抗原表位。结论利用生物信息学预测出的结构和功能信息,能为PSME蛋白的相关研究提供信息基础。

This paper focuses on the prediction of structures and functions of a new alternatively spliced variant of prostate-specific membrane antigen （PSME） using bioinformafics methods. ExPASy＇s tools such as computer PI/Mw, SMART and other software were used to analysis PSME amino acid sequence. Furthermore, protein interaction network was predicated by STRING, and B-cell epitopes of the protein was predicted with DNA Star. We fund that the protein PI value was 638, Mr was 78 000, A-helix secondary structure （H） accounted for 34.66%, [3 fold （E） accounted for 12.93%, and random coil accounting for 52.41%. PSME signal peptide was in the 1-22 bit, peptidase domain wan in 150-248, and TFR dimer in 639-703 bit. Furthermore, PSME was also contained multiple glycosylation sites. The predication of structures and functions lays down a information foundation for the further investigation of PSME.