肾透明细胞癌与癌旁组织差异蛋白表达谱分析

Proteomic profiling of renal clear cell carcinoma and paratumorous tissues

目的:通过比较肾透明细胞癌和癌旁组织的蛋白表达谱差异,筛选新的肾癌肿瘤标志,为提高肾癌诊断的敏感性和特异性提供基础。方法:分别提取10例肾透明细胞癌及其癌旁肾组织蛋白,蛋白定量后,采用双向凝胶电泳(2-DE)对组织蛋白表达谱进行分离,采用飞行时间质谱对差异位点进行蛋白鉴定。结果:鉴定出20个肾癌上调蛋白,10个下调蛋白,上调蛋白集中于缺氧反应和抗凋亡,以及Glycolysis、Regulation of actin cytoskeleton和PPAR信号通路。结论:本研究筛选出一组在肾癌组织中高表达的蛋白,是肾透明细胞癌的候选肿瘤标志,为进一步的验证和功能研究提供了基础。

OBJECTIVE： To compare the proteomic profiling of renal clear cell carcinoma （RCC） and paratumorous tissues, in order to identify novel tumor markers. METHODS： Protein samples from 10 RCC tissues and their paired paratumor tissues were extracted, and isolated by two-dimensional electrophoresis. Differently expressed protein spots were identified by mass spectrometry. RESULTS： About 20 up-regulated and 10 down-regulated proteins in RCC tissues. Most of the up-regulated proteins were involved in response to hypoxia, anti-apoptosis and Glycolysis, Regulation of actin cy-toskeleton and PPAR cancer-related pathway. CONCLUSION： The differently identified proteins in this study were novel candidate biomarkers for RCC, and its clinical significance deserves further investigation.