子宫内膜癌组织NRF-1和mtTFA表达及其临床意义的初步分析

Preliminary analysis of the expression and significance of NRF-1 and mtTFA in endometrial carcinoma tissues

目的:探讨核呼吸因子1(NRF-1)与线粒体转录因子A(mtTFA)在子宫内膜癌组织中表达的相关性研究。方法:采用SP免疫组化染色法检测NRF-1与mtTFA蛋白在41例子宫内膜癌组织和23例非典型增生子宫内膜组织中的表达,同时选择21例正常子宫内膜组织作为对照。结果:NRF-1和mtTFA在子宫内膜癌中的表达阳性率均明显高于正常子宫内膜组织和非典型增生子宫内膜组织,从正常子宫内膜组织到非典型增生内膜组织,再到子宫内膜癌组织,其表达阳性率均呈逐渐上升趋势,差异有统计学意义,χ2=24.564,P=0.000 1;χ2=33.372,P=0.000 1。NRF-1和mt-TFA的表达与临床分期、组织学分级、肌层浸润及淋巴结转移情况均有明显相关性,且差异有统计学意义,P<0.05。子宫内膜癌组织中mtTFA与NRF-1的表达呈正相关,rs=0.806,P=0.004。结论:NRF-1和mtTFA蛋白的表达异常可能在子宫内膜癌变过程中产生一定的作用。

OBJECTIVE：To investigate the expression of NRF-1 and mtTFA in the endometrial carcinoma tissue. METHODS：Expression of NRF-1 and mtTFA was examined in 41 cases of endometrial carcinoma tissue and 23 cases of a- typical hyperplasia endometrial tissue and in 21 normal endometrial tissue as control groups by SP immunohistochemical staining. RESULTS：The expression of NRF-1 and mtTFA in the endometrial carcinoma were significant higher than in that the normal uterus endometrial tissue and atypical hyperplasia endometrial tissue. From normal tissue to atypical hy-perplasia endometrium and to carcinoma tissue, there was a gradual increase in both of two factors＇ expression（X^2= 24. 564, P= 0. 000 1 X^2= 33. 372, P= 0. 000 1）. And their expressions were clearly correlated with clinical stages, histological grading, muscle invasive and lymphaticmetastasis status（P〈0. 05）. There was positive correlation between the expression of mtTFA and NRF-1 in the endometrial carcinoma tissue（rs=0. 806, P= 0. 004）. CONCLUSION：Based on the results of this study, the abnormal expression of NRF-1 and mtTFA may play roles in the carcinogenesis of endometrial cancer.