骨肉瘤组织凋亡诱导因子与波形蛋白表达及其临床意义的探讨

Expression and clinical significance of AIF and VIM in human osteosarcoma tissues

目的:探讨凋亡诱导因子(AIF)与波形蛋白(VIM)在人骨肉瘤组织中的表达及对骨肉瘤的诊断和预后评估的价值。方法:采用免疫组化技术检测45例骨肉瘤、30例骨软骨瘤的AIF与VIM的表达。结果:AIF和VIM在骨肉瘤细胞中表达阳性率分别为66.70%(30/45)和73.81%(33/45),AIF与VIM在骨软骨瘤组织中低表达;AIF和VIM在骨肉瘤组织中表达无显著相关性,P>0.05;AIF与VIM的表达与性别、年龄、病程、肿瘤体积、分化程度以及生存状况均无关,P>0.05。AIF的表达与骨肉瘤瘤的转移无关,P>0.05,VIM的表达与骨肉瘤的转移相关,P<0.05。结论:AIF和VIM与骨肉瘤的发生密切相关,是评价骨肉瘤的重要指标。

OBJECTIVE： To study the association of apoptosis inducing factor （AIF） and vimentin （VIM） in osteo- sarcoma with diagosis, metastasis and prognosis. METHODS： Microwave SP-immunohistochemical method was used to detect 45 cases of osteosarcoma in the expression of AIF and VIM, combined with the biological behavior of osteosarcoma and clinical follow-up data for analysis and evaluation. At the same time with 30 cases of benign tumor of bone cartilage as a negative control. RESULTS： AIF in osteosarcoma and VIM expression rates were 66. 70% （30/45） and 73. 81% （33/45）. AIF and VIM was weakly expressed in benign tumor of bone cartilage with no statistical significance between both group（P〉0.05）. AIF and VIM expression in osteosarcoma was not related to sex, age, progress, differentiation, lymph node metastasis（P〉0.05）. VIM was related to metastasis（P〈0.05）, but AIF not（P〉0.05）. CONCLUSION： Expression of AIF and VIF is related to, and main factors to evaluate detection of expression of AIF and VIM, and the prognosis of osteosarcoma.