Cox-2在舌鳞癌细胞Tca8113增殖机制中的作用

The Role of Cox-2 in the Proliferation of Squamous Cells of Human Tongue Cancer(Tca8113)

目的:观察并探讨Cox-2在舌鳞癌细胞(Tca8113)增殖机制中的作用。方法:通过免疫细胞化学检测Cox-2在Tca8113细胞中的表达;再通过建立裸鼠荷瘤模型及应用Cox-2特异性抑制剂(SC236)进行干预的方法,观察SC236对Tca8113细胞的增殖活性、致瘤性和成瘤速度的影响。结果:在Tca8113细胞中存在Cox-2的表达;将Tca8113细胞接种于裸鼠颈部皮下可成功诱导出组织学形态与人舌鳞癌基本一致、且表达Cox-2的移植瘤。SC236能够显著抑制Tca8113细胞及其诱导的移植瘤的生长(P<0.01),并明显延缓Tca8113细胞的成瘤速度(P<0.05)。结论:Cox-2在Tca8113细胞的增殖过程中可能发挥重要作用;Cox-2特异性抑制剂可显著抑制Tca8113细胞及其诱导的移植瘤的增殖和生长。

Objective： To observe and explore the role of Cox-2 in the cell proliferation of squamous cells of hu- man tongue cancer （Tca8113 cell）. Methods： Immuocytochemistry was used to detect the expression of Cox-2 in the Tca8113 cells in vitro. Then, methods of intervention on Tca8113 cells with SC236 （specific inhibitor of Cox-2）, and establishment of athymie mouse model bearing cancer, were used to observe the impact of SC236 on the proliferation, oncogenicity, and velocity of tumorigenesis of Tca8113 cell. Results： Cox-2 was confirmed to be ex- pressed in the Tca8113 cells by imuocytoehemistry. By using Tea8113 cell, transplantation tumor, which was es- sentially consistent with the human squamous ceil cancer of tongue histologically and positive to Cox-2, was suc- cessfully adduced. And SC236, exhibited the significant effect of inhibiting the proliferation activity and the velocity of tumorigenesis of Tca8113 cells, and the growth of the developed transplantation tumor adduced from Tca8113 cell. Conclusion： Cox-2 probably played key role in the proliferation of Tca8113 cell. SC236, a specific inhibitor of Cox-2, had the significant effect of inhibiting the proliferation of Tca8113cell and the growth of transplantation tumor adduced from Tca8113 cell.