miR-150对降植烷诱导的狼疮鼠肺出血的影响

Influence of miR-150 on pulmonary hemorrhage in pristane-induced murine lupus model

本研究旨在观察miR-150在降植烷诱导的狼疮鼠中对肺出血的发生和严重程度的影响并初步探讨其可能的机制。实验选用8周龄C57/B6小鼠和miR-150基因缺陷小鼠,腹腔注射降植烷(pristane),在注射后的第7天、第10天、第14天观察肺脏大体病理变化,苏木素-伊红(HE)染色观察肺组织病理变化并进行病理评分。发现注射降植烷第10天和(或)第14天后C57/B6组和miR-150缺陷小鼠组均发生不同程度的肺出血,后者的严重程度大于前者。肺组织病理评分显著升高;miR-150缺陷组与C57/B6组比较血浆TNF-α表达升高,右肺上叶组织湿干比值(W/D)也显著升高。提示在降植烷诱导的狼疮鼠模型中miR-150参与了肺出血的发病过程,miR-150有望成为预测狼疮肺出血的生物标记物。

To investigate the effect of miR-150 on pulmonary hemorrhage in pristane-induced murine lupus model and to explore the possible mechanism,C57/B6 mice and miR-150-deficient mice received a single dose of 0.5 ml pristane intraperitoneally at eight-weeks old.After 7 days,10 days,14 days,pulmonary hemorrhage was investigated by gross pathology and hematoxylin-eosin stain scores.We found that both C57/B6 group and miR-150-deficient group suffered pulmonary hemorrhage,and miR-150-deficient group was more serious than C57/B6 group 10 days and 14 days after injection of pristane.In addition,compared to C57/B6 group,its histological score is higher.Then,the quantification of TNF-α in serum was performed by sandwich enzymed-linked immune absorbent assay（ELISA） and the right upper lobe of the lung was weighted before and after drying.Results showed that expression of TNF-α in the serum and W/D ratio of miR-150-deficient group was higher revealing that miR-150 participates the pathogenesis of pulmonary hemorrhage in pristane-induced murine lupus model and hopefully be a forcasting biomarker of pulmonary hemorrhage of lupus.