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B7-H3在类风湿关节炎患者滑膜组织内的表达及分布研究 被引量：1

Expression and distribution of the costimulatory molecule B7-H3 in synovial tissues from rheumatoid arthritis
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摘要 探讨B7家族共刺激分子B7-H3在类风湿关节炎(RA)患者滑膜组织内的表达及分布。方法:使用免疫组化法检测RA患者滑膜组织B7-H3的表达,并使用免疫荧光法检测B7-H3在细胞中的定位及分布。结果:免疫组化结果证实,RA患者滑膜组织中有大量的B7-H3阳性细胞,形态观察提示这些阳性的细胞主要为毛细血管细胞、滑膜细胞、局部淋巴结处的T细胞及巨噬细胞;免疫荧光分析进一步表明这些B7-H3+细胞主要为CD68+巨噬细胞,CD31+内皮细胞及CK-18+上皮细胞。此外,对比其他B7家族共刺激分子在滑膜组织中的分布,免疫荧光发现B7-H3共表达于B7-DC+及HVEM+血管,但不表达于B7-H1+及B7-H4+细胞。类风湿关节炎滑膜组织内有大量B7-H3阳性细胞,提示B7-H3有可能参与并调节关节炎的病理进程。 To detect the expression of B7-H3 antigen in synovial tissues from rheumatoid arthritis（RA） patients,the expression and distribution of B7-H3 antigen was analyzed by immunohistochemistry.Moreover,fluorescence double-staining was further used to characterize the cell type which express B7-H3.Immunohistochemical analysis revealed that B7-H3 positive cells were found in RA synovium,and fluorescence double-staining further demonstrated that B7-H3 positive cells were CD68^＋ macrophages,CD31^＋ endothelial cells as well as CK-18＋ epithelial cells.In contrast to other members of the B7 superfamily,the expression of B7-H3 was also found on B7-DC^＋ as well as HVEM＋ capillary,but not on B7-H1^＋ and B7-H4^＋ cells.The expression of B7-H4 was observed on the surface of several kinds of cell types within synovial tissues of RA patients,indicating that this signal might be involved in regulating local T cell activation and finally participates pathogenesis of RA.
作者 尚永军 崔其福 李金龙 郭国宁
机构地区 内蒙古赤峰学院第一附属医院骨科 第三军医大学附属西南医院急诊科
出处 《现代免疫学》 CAS CSCD 北大核心 2012年第3期205-209,共5页 Current Immunology
基金 国家自然科学基金资助项目(60971117 61141012)
关键词 B7-H3 类风湿关节炎 滑膜组织 免疫组化 B7-H3 rheumatoid arthritis synovial tissues immunohistochemistry
分类号 R392.3 [医药卫生—免疫学]
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参考文献14

  • 1Goekoop-Ruiterman YP, Huizinga TW. Rheumatoid arthri- tis: ten we achieve true drug-free remission in patients with RA[J]. Nat RevRheumatol, 2010,6:68-70.
  • 2McInnes IB, Schett G. Cytokines in the pathogenesis of rheu- matoid arthritis[J]. Nat Rev Immunol, 2007,7,429-442.
  • 3Gary SF. Evolving concepts of rheumatoid arthritis[J]. Na ture, 2003,423: 356-361.
  • 4SharpeAH, Abbas, AK. T cell eostimulation biology, thera peutic potential, and challenges[J]. N Engl J Med, 2006, 355:973- 975.
  • 5Kremer JM, Westhovens R, Leon M, et al. Treatment of rheumatoid arthritis by selective inhibition of T cell activation with fusion protein CTI.A4Ig[J]. N Engl J Med, 2003,349: 1907-1915.
  • 6Pierer M, Schulz A, Rossol M, et al. Herpesvirus entry me diator-Ig treatment during immunization aggravates rheuma- toid arthritis in the collagen induced arthritis model[J]. J Im- munol, 2009,182: 3139-3145.
  • 7Hu YL, Metz DP, Chung J, etal. /37RP-1 blockade ametio- rates autoimmunity through regulation of follicular helper T cells[J]. 1 lmmunol. 2009.182,1421142-1421148.
  • 8Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-garnma production[J]. Nat Immunol, 2001,2:269-274.
  • 9Steinberger P, Majdie O, Derdak SV, et al. Molecular char- acterization of human 4Ig BT-H3, a member of the B7 family with four Ig-like domains[J]. J Immunol, 2004,172: 2352-2359.
  • 10Yi KH, Chen L. Fine tuning the immune response through B7-H3 and B7-H4[J]. ImmunolRev, 2009,229:145-151.

二级参考文献11

  • 1Freeman GJ, Long AJ,Iwai Y,et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation[J]. J Exp Med,2000,192:1027.
  • 2Dong H,Zhu G,Tamada K,et al. B7-H1,a third member of the B7 familiy,co-stimulates T-cell proliferation and interleukin-10 secretion[J]. Nat Med,1999,5:1365.
  • 3Yoshinaga SK,Whoriskey SD,Khare SD,et al. T cell co-stimulation through B7RP-1 and ICOS[J]. Nature,1999,402:827.
  • 4Chapoval AI,Ni J,Lau JS,et al. B7-H3:a costimulatory molecule for T cell activation and IFN-gamma production[J]. Nat Immunol,2001,2:269.
  • 5Ling V,Wu PW,Spaulding V,et al. Duplication of primate and rodent B7-H3 immunoglobulin V-and C-like domains:divergent history of functional redundancy and exon loss[J]. Genomocs,2003,82:365.
  • 6Suh WK,Gajewska BU,Okada H,et al. The B7 family member B7-H3 preferentially down-regulates T helper type 1-mediated immune responses[J]. Nat Immunol,2003,4:899.
  • 7Sun X,Vale M,Leung E,et al. Mouse B7-H3 induces antitumor immunity[J]. Gene Ther,2003,10:1728.
  • 8Steinberger P,Majdic O,Derdak SV,et al. Molecular characterization of the human 4Ig-B7-H3,a member of the B7 family with four Ig-like domains[J]. J Immunol,2004,172:2352.
  • 9Liang L,Sha WC. The right place at the right time:novel B7 family members regulate effector T cell responses[J]. Curr Opin Immunol,2002,14:384.
  • 10Bretscher PA,Coln M.A theory of self and non-self discrimination[J]. Science. 1970,169:1042.

共引文献8

同被引文献15

  • 1Wan B, Nie H, Liu A, et al. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis [J]. J Immunol, 2006, 177(12) : 8844 -8850.
  • 2MareUi-Berg FM, Okkenhaug K, Mireuda V. A two-signal model for T cell trafficking[J]. Trends Immunol, 2007, 28(6) : 267 -273.
  • 3Watts TH. TNF/TNFR family members in cosfinatlafion of T cell responses [J]. Annu Rev Immunol, 2005, 23:23-68.
  • 4Greenwald RJ, Freeman GJ, Sharpe AH. The B7 family revisited[ J]. Annu Rev Immunol, 2005, 23:515-548.
  • 5Sharpe All, Freeman GJ. The B7-CIY28 superfamily[J]. Nat Immunol, 2002, 2(2) : 116-126.
  • 6Coyle AJ, Gutierrez-Ramos JC. Increasing complexity in costimulatory signals regulating T cell function[J]. Nat Rev Immunol, 2001, 2(3): 203-209.
  • 7Chapoval AI, Ni J, Lau JS, et al. BT-I-D: A costimulatory molecule for T cell activation and IFN-gamma production[ J]. Nat Immunol, 2001, 2(3) : 269 -274.
  • 8Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes [ J ]. J Immunol, 2002, 168 ( 12 ) : 6294 - 1697.
  • 9Zhang GB, Zhou H, Chen YJ, et al. Characterization and application of two novel monoclonal antibodies against 21gB7-I-I3: expression analysis of 2IgBT-H3 on dendritic cells and tumor cells [ J ]. Tissue Antigens, 2005, 66 (2) : 83 - 92.
  • 10Steinberger P, Majidic O, Derdar SV, et al. Molecular characterization of human 4Ig-B7-I-D, a member of 137 family with four Ig-like domains [J]. J Irmnunol, 2004, 172(4) : 2352 -2359.

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现代免疫学
2012年 第3期

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