cAMP反应元件结合蛋白在高糖所致内皮细胞损伤中的作用

Role of cAMP Response Element Binding Protein in Endothelial Cells Injury Induced by High Glucose

目的探讨cAMP反应元件结合蛋白(CREB)在高糖致内皮细胞损伤中的作用。方法建立高糖诱导的血管内皮细胞损伤模型,将内皮细胞ECV304分为对照组、高糖组、转染PCI组、转染PCI+高糖组、转染PCI-CREB组和转染PCI-CREB+高糖组,采用MTT法检测细胞存活率,流式细胞仪检测细胞凋亡率,硫代巴比妥酸法检测丙二醛(MDA)含量,黄嘌呤氧化酶法检测细胞超氧化物歧化酶(SOD)活性。结果转染PCI-CREB组和转染PCI组内皮细胞存活率、SOD活性、MDA含量和细胞凋亡率与对照组相比差异无统计学意义(P>0.05);与对照组相比,高糖组内皮细胞存活率和SOD活性明显下降,而MDA含量和细胞凋亡率明显增加(P<0.05);转染PCI+高糖组内皮细胞存活率、SOD活性、MDA含量和细胞凋亡率与高糖组相比差异无统计学意义(P>0.05);与高糖组相比,转染PCI-CREB+高糖组细胞存活率和SOD活性明显高于高糖组(P<0.05),而MDA含量和细胞凋亡率明显低于高糖组(P<0.05)。结论过表达CREB对高糖诱导的内皮细胞损伤具有保护作用。

Aim To investigate the role of cAMP response element binding protein（CREB） in endothelial cells injury induced by high glucose.Methods The endothelial cell injury model was induced by high glucose.ECV304 cells were divided into six groups： control group,high glucose group,PCI transfection group,PCI transfection＋high glucose group,PCI-CREB transfection group and PCI-CREB transfection＋high glucose group.The cell survival rate of ECV304 cells was detected by MTT method.Flow cytometry was used to detect the apoptosis rate.The MDA concentration was observed by TBA method and SOD activity was tested by xanthine oxidase method.Results Compared with control group,cell survival rate,SOD activity,MDA concentration and apoptosis rate in PCI-CREB transfection group and PCI transfection group were not significantly different（P0.05）.Compared with control group,both cell survival rate and SOD activity in high glucose group showed a significant decrease,however,apoptosis rate and MDA concentration increased significantly（P0.05）.Cell survival rate,SOD activity,MDA concentration and apoptosis rate in PCI transfection＋high glucose group was not significantly different compared with high glucose group（P0.05）.Compared with high glucose group,both cell survival rate and SOD activity in PCI-CREB transfection＋high glucose group showed a significant increase,however,apoptosis rate and MDA concentration decreased significantly（P0.05）.ConclusionOverexpression of CREB may protect endothelial cells from high glucose injury.