软肝宁的抗大鼠肝纤维化作用

Effects of ruanganning against liver fibrosis in rats

目的探讨软肝宁对四氯化碳(CCl4)所致肝纤维化大鼠的影响。方法 60只雄性Wister大鼠均分为六组:除正常对照组外,其余五组采用CCl4复制肝纤维化大鼠模型后分为模型对照组、软肝宁高、中、低剂量组(软肝宁0.12,0.06,0.03g/kg)和秋水仙碱组。给药6周后检测大鼠血清ALT、AST、超氧化物歧化酶(SOD)、丙二醛(MDA)、总蛋白(TP)、白蛋白(ALB)及羟辅氨酸(Hyp),并行大鼠肝组织常规病理检查。结果模型对照组的ALT、AST、MDA和Hyp均高于正常对照组,而SOD、TP及ALB活性低于正常对照组(P<0.05或P<0.01)。软肝宁高、中剂量组的ALT、AST、MDA和Hyp均低于模型对照组,而SOD、TP及ALB活性均高于模型对照组(P<0.05或P<0.01)。病理检查显示,软肝宁中、高剂量组大鼠的肝组织损害明显比模型对照组轻。结论软肝宁具有延缓CCl4所致肝纤维化进程的作用。

Objective To study the effects of ruanganning（a compound of Chinese medicine） on liver fibrosis induced by carbon tetrachloride（CCl4） in rats. Methods Sixty male Wister rats were randomly divided into six groups with 10 rats each.The rat models of hepatofibrosis were produced by subcutaneous injection of CCl4 in 50 rats,which were equally divided into 5 groups of MC（model control）,R1（treated with ruanganning 0.12 g/kg）,R2（treated with ruanganning 0.06 g/kg）,R3（treated with ruanganning 0.03 g/kg） and PC（treated with colchicines as positive control medicine）.The rest 10 rats were taken as blank controls（group BC）.Six weeks later,serum levels of ALT,AST,TP,ALB,and Hyp and the activities of SOD and MDA in the liver were detected and the liver specimen was examined pathologically. Results The levels of ALT,AST,MDA and Hyp were higher,but those of SOD,Tp and ALB were lower,in group MC than those in group BC（P0.05 or P0.01）. The levels of ALT,AST,MDA and Hyp were lower,but those of SOD,Tp and ALB were higher,in groups of R1 and R2 than those in group MC（P0.01 or P0.05）.Pathologically,the severity of liver damage was slighter in groups of R1 and R2 than that in group MC. ConclusionRuanganning is capable of inhibiting hepatofibrosis in rats model.