VCAM-1和MCP-1在实验性变态反应性脑脊髓炎大鼠模型视神经内的表达

Expression of VCAM-1 and MCP-1 in optic nerve of experimental allergic encephalomyelitis in rat

目的观察实验性变态反应性脑脊髓炎大鼠模型视神经内血管内皮细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)的表达变化,为研究视神经炎(optic neurikis,ON)的发病机制提供新的实验依据。方法用豚鼠脑脊髓匀浆和完全弗氏佐剂诱导Wistar大鼠,建立实验性变态反应性脑脊髓炎模型,将50只Wistar大鼠随机分为对照组和免疫后7d、12d、18d、25d组,通过行为学观察对各组进行神经功能评分,通过HE染色法观察各组视神经的病理形态学改变,采用免疫组织化学染色,观察各组大鼠VCAM-1和MCP-1的表达情况。结果神经功能评分显示,与对照组相比,免疫组大鼠随着时间的延长,神经功能评分增加,至18d,达到最大的1.9分。HE染色结果显示,对照组大鼠视神经组织结构正常,而免疫组大鼠视神经组织出现结构的异常,免疫后18d,病变程度达最高峰,这和功能评分的结果相符。免疫组织化学结果显示,对照组没有VCAM-1和MCP-1表达;免疫后7d,VCAM-1和MCP-1开始表达,阳性细胞数分别为11.45±8.65和47.86±9.65;免疫后12d,VCAM-1和MCP-1表达均进一步增加,阳性细胞数分别为17.34±5.76和86.45±5.61,与对照组比较差异均有统计学意义(均为P<0.05);免疫后18d,VCAM-1和MCP-1表达达到高峰,阳性细胞数分别为32.53±9.56和153.56±12.47,与对照组比较差异均有显著统计学意义(均为P<0.01);免疫后25d,VCAM-1和MCP-1表达减少,阳性细胞数分别为21.23±7.58和116.49±8.83。结论 VCAM-1和MCP-1参与了ON的发病过程,ON是一种免疫因素介导的疾病。

Objective To observe the expression of vascular cell adhesion molecule-1（VCAM-1） and monocyte chemoattractant protein-1（MCP-1） in optic nerve of experimental allergic encephalomyelitis（EAE） in rat,and provide new experimental evidence for pathogenesis of optic neurikis（ON）.Methods EAE was induced with GPSCH and CFA,50 healthy Wistar rats were randomly divided into control group and 7 days,12 days,18 days,25 days after immunization group,the clinical neurological function scores were observed by behavior observation,and the pathological changes of optic nerve were observed by HE staining,expression of VCAM-1 and MCP-1 in each group was detected by immunohistochemistry.Results Compared with the control group,the clinical neurological scores showed that the function scores of immunization groups began to rise with the time prolong,the maximum score was 1.9 at 18 days.HE staining showed that the structure of optic nerve in immunization groups appeared abnormal,the lesions became more severely at 18 days after immunization,which was conformed to the result of function score.Immunohistochemistry demonstrated that in control group,the expression of MIP-1 and ICAM-1 was not detected,but in immunization groups,the expression of MIP-1 and ICAM-1 began to exist at 7 days after immunization,the positive cell numbers were 11.45±8.65 and 47.86±9.65,increased at 12 days,which were 17.34±5.76 and 86.45±5.61,there were statistical differences compared with control group（both P0.05）;The expression of MIP-1 and ICAM-1 peaked at 18 days after immunization,the positive cell numbers were 32.53±9.56 and 153.56±12.47,there were significant differences compared with control group（both P0.01）;The expression of MIP-1 and ICAM-1 began to decrease at 25 days after immunization,the positive cell numbers were 21.23±7.58 and 116.49±8.83.Conclusion MIP-1 and ICAM-1 may be involved in the pathogenesis of ON,which is a disease caused by immunity factors.