摘要
目的观察血管紧张素转换酶2(ACE2)在自发性高血压大鼠(SHR)左心室中的表达以及缬沙坦干预后对ACE2表达水平的影响。方法 24只12周龄雄性SHR随机分为SHR组、缬沙坦组,12只同龄雄性血压正常的Wistar大鼠作为正常对照组。10周后处死,测定心脏重量指数(HWI)、左心室重量指数(LVWI);酶联免疫法测定血浆中AngⅡ的浓度;碱水解法测定心肌中羟脯氨酸的含量;反转录-聚合酶链反应检测心肌中ACE2的表达。结果与正常对照组比较,SHR组LVWI、血浆中AngⅡ的浓度以及心肌羟脯氨酸的含量均增高(P<0.05),心肌组织中ACE2的表达显著降低(P<0.05);与SHR组比较,缬沙坦组LVWI、血浆中AngⅡ的浓度以及心肌羟脯氨酸的含量均降低(P<0.05),心肌组织中ACE2表达显著增高(P<0.05)。结论缬沙坦可逆转高血压左心室重构,机制可能与增加心肌中ACE2的表达有关。
Objective To investigate the expression of angiotensin converting enzyme 2（ACE2） in the SHR,and the effect of valsartan on the expression of ACE2 mRNA.Methods Twenty-four Twelve-week-old male SHR were randomly divided to SHR group and valsartan group;Twelve-week-old male Wistar rats were served as control group.After 10 weeks,determine the heart weight index and the 1eft ventricular weight index;determine the concentration of AngⅡ in plasma by ELISA;determine the hydroxyproline content in myocardium by buck solution;The expression of ACE2 mRNA in myocardium was measured by RT-PCR.Results Compared with the control group,the LVWI,the hydroxyproline content and the concentration of AngⅡ were significantly increased in the SHR group,but the ACE2 expression was markedly decreased;compared with SHR group,the above indexes were decreased except for ACE2 was increased in valsartan group.Conclusions Valsartan can reverse left ventricular remoding in hypertension.The possible mechanism is that valsartan can incerease the expression of ACE2.
出处
《基础医学与临床》
CSCD
北大核心
2012年第6期678-681,共4页
Basic and Clinical Medicine
基金
山东省医药科技发展计划(2009HW008)
