多发性肌炎外周血白细胞中细胞因子信号转导蛋白抑制因子基因表达的初步研究

Preliminary Research on the Expression of Suppressor of Cytokine Signaling in Peripheral Blood Leukocytes of Polymyositis Patients

目的研究活动期多发性肌炎患者外周血白细胞细胞因子信号转导蛋白抑制因子(SOCS)1、SOCS2、SOCS3和细胞因子诱导的含SH2区域蛋白1(CIS)与正常人表达的差异,探讨SOCS在多发性肌炎发病中可能的作用。方法 2011年6月-12月,采用实时荧光定量聚合酶链反应法检测了14例活动期多发性肌炎患者和14例正常人外周血白细胞中SOCS1、SOCS2、SOCS3和CIS1基因的相对表达量。结果与对照组相比,多发性肌炎症患者外周血白细胞基因SOCS 1～3表达明显降低(P值均<0.05),CIS1基因的表达较对照组明显升高(P<0.05),差异有统计学意义。结论 SOCS基因家族可能参与了多发性肌炎的发病,该蛋白分子家族的成员可能会成为多发性肌炎治疗的一种新的候选基因。

Objective To investigate the difference of expression of suppressor of cytokine signaling（SOCS）（SOCS1,SOCS2,SOCS3） and cytokine-inducible SH2 containing protein 1（CIS1） in peripheral blood between polymyositis（PM） patients and healthy controls,and explore the possible roles of SOCS in the pathogenesis of PM.Methods From June to December 2001,peripheral blood samples from patients with PM（n=14） were collected and blood samples from 14 healthy adults were used as control.All the patients enrolled in this study were at active course of PM.The expression of SOCS 1-3 and CIS1 mRNA in peripheral blood leukocyte（PBL） from PM patients and healthy controls were quantified by real-time PCR analysis.Results The mRNA levels of SOCS 1-3 in PBL of PM patients were significantly lower than those in the healthy controls（P〈0.05）.In contrast,CIS1 expression was significantly higher in PBL of PM patients in comparison with its expression in the healthy controls（P〈0.05）.Conclusions SOCS mRNA expression levels are profoundly altered in PM patients,and SOCS cytokines may play an important role in the pathogenesis of PM.SOCS family may be a new candidate gene in the therapy of PM.