RUNX3与Survivin在子宫内膜癌中的表达

Expression of RUNX3 and Survivin in Endometrial Carcinoma

目的探讨RUNX3和Survivin在子宫内膜癌(endometrial carcinoma,EC)组织中的表达。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法(streptavidin-peroxidase,SP)检测20例正常增生期子宫内膜、20例非典型增生子宫内膜、60例EC组织中RUNX3和Survivin蛋白的表达,比较其差异。结果在EC中,RUNX3和Survivin蛋白的阳性表达率分别是25.0%(15/60)和91.7%(55/60),随着组织学分级升高及分期进展,RUNX3阳性表达率逐渐降低(χ2=16.275,P=0.000),而Survivin蛋白表达逐渐升高(χ2=6.251,P=0.044),两者阳性表达率均与肌层浸润深度、淋巴结转移及有无脉管浸润无关(P均>0.05)。Survivin蛋白在EC中的阳性表达率91.7%(55/60)明显高于正常增生期15.0%(3/20)和非典型增生子宫内膜65.0%(13/20)(χ2=44.221,P=0.000;χ2=8.848,P=0.000),其中正常增生期与非典型增生子宫内膜Survivin蛋白表达阳性率有统计学差异(χ2=8.640,P=0.003)。RUNX3蛋白在EC中的阳性表达率25.0%(15/60)明显低于正常增生期90.0%(18/20)和非典型增生子宫内膜60.0%(12/20)(χ2=26.151,P=0.000;χ2=8.218,P=0.004),正常增生期与非典型增生子宫内膜RUNX3蛋白阳性表达率无统计学差异(χ2=3.333,P=0.068)。结论 EC中RUNX3蛋白表达较低或缺失,Survivin蛋白表达较高。

Objective To investigate the expression of RUNX3 and Survivin protein in endomctrial carcinoma （EC）. Methods The expression of RUNX3 and Survivin protein in endometrial carcinoma（ EC, n = 60） , atypical endometrial hyperplasia （n = 20） and normal endometrial tissues （n = 20） were examined by immunohistochemistry （SP method）. Results In EC, the positive expression rates of RUNX3 and Survivin were 25.0% （15/60） and 91.7% （55/60） , respectively. With the histological grade increasing and FIGO stage progressing, the positive expression rate of RUNX3 decreased （χ^2=16. 275, P = 0. 000） , and that of Survivin increased （χ^2= 6. 251, P = 0. 044）. The expression of RUNX3 and survivin were not related to the depth of muscular invasion, lymph node metastasis and lymphatic vessel invasion（ all P 〉 0.05）. The positive expression rate of Survivin protein in EC （91.7% ,55/60） was significantly higher than that in normal proliferative endometrium（ 15.0%, 3/20） and atypical endometrial hyperplasia（65.0% ,13/20）（χ^2= 44. 221, P = 0. 000; χ^2= 8. 848, P = 0. 000）, and between normal endometrium and atypical endometrial hyperplasia, the positive expression rate of Survivin protein was also significant different （χ^2= 8. 640, P = 0. 003 ）. Whereas, the positive rate of RUNX3 in EC （25.0% , 15/60） was significantly lower than that in normal proliferative endometrium （90.0% , 18/20） and atypical endometrial hyperplasia （60.0% , 12/20） （χ^2= 26. 151, P = 0. 000; χ^2= 8. 218, P = 0. 004 ）, and between normal proliferative endometrium and atypical endometrial hyperplasia, the positive expression rate of RUNX3 protein was not significantly different（χ^2= 3. 333, P = 0. 068 ）. Conclusion The expression of RUNX3 protein is lower or absent in EC, but the expression of Survivin protein is higher.