糖皮质激素注射建立小鼠行为抑郁症模型

A mouse model of behavioral depression induced by repeated corticosterone injections

目的研究糖皮质激素注射小鼠抑郁症样情感行为变化。方法C57/B6雄性小鼠18只随机分为3组,模型组以20 mg/kg剂量每日1次注射皮质酮,持续21 d,另有生理盐水对照组每日注射等剂量生理盐水21 d,空白对照组不做任何处置,观察小鼠体重变化,用敞箱实验及糖水消耗试验测定动物行为。结果抑郁模型组小鼠代表焦虑行为的敞箱试验得分明显降低、反应快感行为的奖赏糖水消耗百分比明显降低。结论糖皮质激素持续注射法可用于制作行为小鼠抑郁模型,以及探究抑郁症行为变化发病机制以及寻找更适合治疗抑郁症的药物。

Objective To explore depression-like Emotional behavioral induced by repeated corticosterone injection.Methods C57/B6 male mice were divided into 3 groups.The model group received repeated injections of vehicle and 20 mg/kg of corticosterone for 21 days,while the saline control group injected daily dose of saline solution for 21 days,blank control group without any treatment.Thenwe bserved the body weight change of mice,used open-field test and sugar consumption test to determine the behaviors of these animals.Results Compare with the control group,the mice showed reduced squares crossing in the open-field test which reflect anxiety behavior,and significantly reduced preference in sucrose preference test which was a reaction behavior of the reward.The mice model of depression had been established successfully.Conclusion Glucocorticosteroid continuous injection method can successfully establish a reliable mouse behavioral depression model,and can be used to further explore the pathogenesis behavioral variation of depression as well as looking for drugs in the treatment of depression.