摘要
目的分析探讨儿童白细胞介素-1(IL-1)基因簇多态性与幽门螺杆菌(H.pylori)感染间的相关性。方法随机选取128例消化道疾病患儿的胃黏膜标本。IL-1B-31,IL-1B-511SNP位点多态性使用实时定量聚合酶链(real-time PCR)双色荧光探针法检测,IL-1RN的可变重复序列使用传统PCR方法检测。Real-time PCR检测H.pylori的保守基因ure以判断H.pylori的感染,高毒力亚型基因vacA s1用Real-time PCR检测,用PCR扩增另一高毒力基因cagA羧基端EPIYA基因序列所在区,然后测序确定其亚型。结果所研究的患儿中IL-1B-31T与IL-1B-511C完全连锁。未检测到H.pylori及其高毒力亚型感染与IL-1-511/-31SNP及IL-1RN基因多态性之间的相关性。结论 IL-1基因簇多态性不是广州地区儿童H.pylori感染的独立影响因素,它可能与其他因素共同影响着儿童H.pylori的感染。
Objective To investigate the relationship between IL-1 gene clusters polymorphisms and H. pylori infection in children. Methods 128 gastric mucosa samples from children with peptic symptoms were randomly selected. Polymorphisms of IL-1B-511 and IL-1B-31 were identified by dual fluorescence PCR. Variable number of tandem repeat region（VNTR） in IL-1RN was detected by conventional PCR. Real-time PCR assay was used to diagnose Helicobacter（H. ） pylori ureA gene and vacA s1 gene. The conserved gene ureA was used to detect H. pylori infection. EPIYA motifs in the 3＇ region of CagA were detected by conventional PCR and DNA sequencing. Results The IL-1B-31T and IL-1B-511C were completely linked in our research. There was no relationship between H. pylori or its high virulence genotypes and SNP of IL-1-511/-31 or polymorphisms of IL-1RN gene. Conclusion IL-1 gene cluster polymorphisms are not the independent influential factors for H. pylori infection in children in Guangzhou area, which may act with other factors in common to influence H. pylori infection in children.
出处
《检验医学与临床》
CAS
2012年第11期1281-1284,共4页
Laboratory Medicine and Clinic
基金
广州医学院博士启动项目(2010C26)
