乙型肝炎病毒DNA水平与血清标志物的探讨分析

Analysis of relationship between HBV-DNA level and serum markers

目的研究乙型肝炎血清学标志物(HBV-M)结果与HBV-DNA水平之间的关系。方法对该院2011年1～12月437例门诊和住院患者血清用荧光定量聚合酶链反应和酶联免疫吸附试验(ELISA)检测,并对结果进行分析。结果437例标本中,其中有136例乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒e抗原(HBeAg)、乙型肝炎病毒核心抗体(抗-HBc)阳性,其血清中HBV-DNA的阳性率为98.5%,含量为7.07±1.31;有175例HBsAg、乙型肝炎病毒e抗体(抗-HBe)、抗-HBc阳性,其血清中HBV-DNA的阳性率为72.0%,含量为5.27±1.46;有67例HBsAg、抗-HBc阳性,其血清中HBV-DNA的阳性率为58.2%,含量为5.11±1.60。结论两种方法检测乙型肝炎标志物有密切的相关性。荧光定量PCR是检测HBV-DNA含量较精确的方法,灵敏度高,特异性强,能正确反映乙型肝炎病毒的复制水平和活跃程度;HBeAg前S1蛋白(PreS1)等血清学标志和HBV-DNA定量测定是乙型肝炎病毒感染的重要方法。

Objective To analyze the relationship between the level of HBV- DNA and HBV-M. Methods 437 outpatients and inpatients in our hospital from January to December 2011 were detected HBV- DNA and HBV-M by fluorescence quantitative PCR and ELISA assay and the results were analyzed. Results Among 437 specimens, 136 cases were positive HBsAg（ ＋）, HBeAg（ ＋ ） and HBcAb（ ＋ ）, and the positive rate of serum HBV-DNA was 98.50/oo ,the content was 7.07±1.31. 175 cases were positive HBsAg（＋） ,HBeAb（＋ ） and HBcAb（＋） ,the positive rate of serum HBV-DNA was 72.0% ,the content was 5.27±1.46.67 cases were positive HBsAg（＋） and HBcAb （4-）, the positive rate of serum HBV-DNA was 58.2 %, the content was 5.11 ±1.60. Conclusion A close correlation exists between these two methods in the detection of HBV markers. The FQ-PCR is a relatively accurate method for detecting HBV -DNA with high sensitivity and strong specificity,which can correctly reflect the replication level and activity degree of HBV. HBeAg, PreS1 and other serum markers and quantitative determination of HBV-DNA are the important method of HBV infection.