饲料中三聚氰胺的HPLC检测方法研究

Study of the Melamine Determination in feed By HPLC

饲料样品前处理时分别考察了提取液用量、沉淀剂用量、提取时间等因素对检测结果的影响,得到最佳前处理条件:提取液为乙腈+水+5%的三氯乙酸(8 mL+14 mL+20 mL),沉淀剂为2 mL,超声提取时间25 min。采用HPLC法测试,外标法定量,方法的检出限为0.2μg/mL,相对标准偏差为2.44%,回收率在88.3%～94.3%之间,20 min内可以完成一个样品的分析测定,建立了一种简便、快捷、准确的测定方法。

In the pre-treatment step of feed samples, the factors were studied to affect on detect consult such as the amount of extraction agent, the amount of precipitant and extraction time. The optimal pre-treatment method was obtained： the extraction agent is acetonitrile-water-5% acetocaustin （8mL＋14mL＋20mL）, the amount of precipitant is 2ml and ultrasound extraction time is 25min. HPLC and ESTD was adopted to determine the content of the melamine in feed. The determination limit was 0.2~tg.mL-~; the relative standard deviation was 2.44% and the mean recoveries were from 88.3% to 94.3%. The determination of one sample is within 20 minutes. A simple, quick and accurate analysis method were established.