湖北省部分地区经血途径感染艾滋病人群中丙型肝炎病毒基因型分布

Epidemiology and distribution of hepatitis C virus genotype among HIV positive former blood donors and transfusion recipients in Hubei province

目的了解湖北省经血途径感染HIV-1人群中合并HCV感染情况及HCV基因型分布。方法2004年7月至2010年12月间在本院诊治或会诊的597例抗HIV阳性者进行HCV筛查，并行HCV病毒载量检测，对HCVRNA阳性者进行逆转录巢式聚合酶链反应扩增HCV核心基因区，并对扩增产物进行测序，采用Mega软件对所得序列进行基因树分析。结果既往有偿供血和受血的HIV感染者中HCV的感染率分别为76．5％（205／268），57．4％（189／329）。97例HIV、HCV合并感染者行HCV基因分型检测，发现1b型90例（92．8％）和2a型7例（7．2％），两型的HCV病毒载量（HCV—VL）差别无统计学意义[对数值分别为（6．0±1．0）拷贝／ml、（5．8±1．4）拷贝／ml，t=0．40，P=0．69]。结论血液途径为HIV、HCV合并感染的主要途径。受血感染者合并HCV感染率低于献血人群，感染者中HCV的基因型主要为1b型和2a型。

Objective To explore the epidemiologic features and distribution patterns of hepatitis C virus （HCV） genotype infection among HIV positive former blood donors （FBDs） and transfusion recipients in Hubei province. Methods 597 serum samples from HIV-positive patients in Hubei were collected and examined for anti-HCV by enzyme-linked immunosorbent assay （ELISA）. Reverse transcription nested polymerase chain reaction （RT-nested PCR） amplification and DNA sequencing were used to evaluate the HCV core regions. Results The prevalence rates of HCV in HIV positive FBDs and transfusion recipients were 76. 5% （205/268） and 57.4% （189/329） respectively. HCV genotypes lb （92. 8%, 90/97 ） and 2a （7.2% , 7/97） were detected. Conclusions Blood donation and blood transfusion are the major modes of HIV-HCV co-infection in Hubei province. The prevalence of HCV in HIV positive transfusion recipients is lower than that in HIV positive FBDs. HCV genotypes lb and 2a are the predominant strains among HIV- positive FBDs and transfusion recipients.