超声萃取-高效液相色谱法测定烟草中β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷

Determination of 3-oxo-α-ionol-β-D-glucopyranoside in tobaccos by high performance liquid chromatography with ultrasonic-assisted extraction

建立了一种用超声波辅助萃取-高效液相法测定烟草中β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷含量的新方法。以甲醇为萃取溶剂,超声萃取条件经过正交实验优化,优化后的条件为料液比1:40(m/V,g/mL)、萃取功率160W,萃取时间20 min。所得萃取液经大孔吸附树脂柱层析法分离后,用Waters SunFireC18(150 mm×4.6 mm,5μm)色谱柱分离,紫外检测器(波长为243nm)检测,流动相为V(乙腈):V(水)=20:80;流速1 mL/min。β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷在0.01～1 mg/mL范围内线性关系良好,相关系数为0.9994,相对标准偏差为1.8%,检出限为0.05μg/mL,平均回收率为87.80%。该方法适用于β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷的定量分析。

a new method for the analysis of 3-oxo -α-ionol β-D-glucopyranoside in ultrasonic assisted extraction products from tobaccos by high performance liquid chromatography （HPLC）was developed. The samples were extracted using methanol and the extraction conditions which were optimized by orthogonal experiment were as followings ： the solid liquid ratio was 1：40, the extraction power was 160W and the extraction time was 20 min. The extraction products from tobaccos were cleaned up by a macroporous resin column and then separated by HPLC. The HPLC was carried out with a Waters SunFire C18 column （150 mm x4. 6 mm, 5 ~xm） and detected by an ultraviolet detector at the wavelength of 243 nm. The acetonitrile-water （20 ： 80, V ： V） was used as the mobile phase with a flow rate of 1.0 mL/min. The linear range for 3-oxo -α--ionol - β-D- glucopyranoside was 0. 01 -1 mg/mL with correlation coefficient of 0. 9994 and the relative standard deviation was 1.8%. The average recovery was 87.80% with the detection limit of 0. 05μg/mL. The method can be applied to the quantitative analysis of 3-oxo -α-ionol - β-D-glucopyranoside in tobacco samples with good stability and repeatability.