分子印迹固相萃取检测罐装食品中双酚A

Molecularly imprinted silica nanospheres for solid-phase extraction of bisphenol A in canned food

以双酚A为模板分子,3-氨基丙基乙氧基硅烷为功能单体,通过溶胶-凝胶反应合成双酚A分子印迹纳米硅胶微球。以印迹微球为固相萃取吸附剂,优化固相萃取条件,确定二氯甲烷为上样溶剂。固相萃取选择性实验表明,在双酚A及其结构类似物四溴双酚A、双酚C、壬基酚的混合物溶液中,印迹萃取柱对双酚A具有良好的选择性能,回收率达到90.7%。浓度为2.5和5μmol/L的加标罐装食品样品,经印迹萃取柱预处理,液相色谱检测得到回收率72%～84%,相对标准偏差2.9%～4.4%。

Molecularly imprinted silica nanospheres with special molecule recognition properties for bisphenol A（BPA） were prepared with a sol-gel process using bisphenol A as a template molecule,and aminopropyltriethoxysilane（APTES） as a functional monomer.The BPA-imprinted silica nanospheres,as the solid-phase extraction（SPE） sorbents,the SPE conditions were optimized,and dichloromethane was selected as the loading solvent.The selective extraction of BPA from BPA and its three structural analogues BPC,TBBPA and NP showed that the BPA-imprinted silica nanoparticles SPE column had much higher selectivity for BPA with recoveries of 90.7%.The canned food samples spiked at two concentration levels of 2.5 and 5 μmol/L BPA were prepared by SPE column and determined by HPLC with recoverey of 72%~84% and relative standard deviations（n= 3） of 2.9%~4.4%.