松质骨基质作为细胞载体应用于牙周组织工程的实验研究

A study on periodontal tissue engineering with cancellous bone matrix as the cell scaffold

目的研究评价松质骨基质(CBM)作为细胞载体应用于牙周组织工程的可行性及意义。方法将体外培养的犬自体牙周膜细胞(PDLCs)接种到CBM三维支架上,扫描电镜观察PDLCs在CBM支架上的生长、附着情况;同时,将PDLCs/CBM复合植入动物牙周组织缺损,以空白组及仅植入CBM组为对照组,术后8周观察评价其牙周组织的再生情况。结果扫描电镜显示PDLCs在CBM上伸展充分,生长旺盛,而CBM具有良好的多孔网状结构。动物实验结果可见PDLCs/CBM复合植入组较对照组有更多的新生牙骨质、新生牙周膜和新生牙槽骨生成,缺损处牙周组织几近完全再生,且未见上皮长入。结论 CBM可作为细胞载体用于牙周组织工程研究,有望用作牙周组织工程的支架材料。

Objective To estimate the feasibility of applying cancellous bone matrix（CBM） as the cell scaffold for periodontal tissue engineering.Methods Dog autogenous periodontal ligament cells（PDLCs） cultured in vitro were collected and seeded on the three-dimensional scaffolds of CBM.The growth of cells on the scaffolds was observed with scanning electronic microscope.Then the PDLCs/CBM composites were transplanted into man-made periodontal defects,and the groups filled with nothing or filled with only CBM were the controls.The dogs were sacrificed 8 weeks later and the periodontium regeneration was observed histologically.Results Scanning electronic microscope showed the flourishing growth of cells on the CBM scaffolds which were in porous structure.The histological observation showed that the PDLCs/CBM group exhibited more new bone,new periodontal ligament and new cementum occupying the majority of the defects than the control groups,and epitheliums were not observed.Conclusions The study proves that CBM can be used as the cell scaffolds for periodontal tissue engineering.